| 摘要: |
| 目的 制备一种光致相变型液态氟碳纳米粒,研究其体外相变及体内增强光声、超声成像能力。方法 采用三步乳化技术制备出以PLGA为载体,液态氟碳(PFH)及吲哚菁绿(ICG)为内核的纳米粒,检测该纳米粒的的粒径、电位,然后体外激光辐照激发纳米粒相变,体内观察该纳米粒增强超声及光声成像的能力。结果 成功制备出包裹PFH和ICG的光致相变纳米粒,为球形,其平均粒径为599.2nm,平均电位为-24.1mv。激光辐照纳米粒时,纳米粒可发生相变转变成微米级的微泡,体内增强了裸鼠移植瘤的光声及超声信号。结论 制备的光致相变型纳米粒在激光作用下发生相变并增强体内超声、光声成像,为临床疾病的诊断提供新思路。 |
| 关键词: 吲哚菁绿 液态氟碳 光致相变 超声成像 光声成像 |
| DOI： |
| 投稿时间：2016-02-25修订日期：2016-02-25 |
| 基金项目:国家自然科学基金青年(81401423、81501484) |
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| Laser-induced phase -shift nanoparticles with ICG and PFH kernel-a dual-modality molecular probeGONG Yuping,CHEN Cheng,SUN Yang,WANG Zhigang |
| gongyuping,wangzhigang |
| (Department of Ultrasound ， the Second Affiliated Hospital of Chongqing Medical University) |
| Abstract: |
| Objective To prepare a laser-induced phase -change liquid fluorocarbon nanoparticle and study on the phase-change in vitro and the enhanced photoacoustic and ultrasound imaging capability in vivo.Methods The nanoparticles with PLGA shell, PFH and ICG kernel were prepared by a three-step emulsification technique. The particle size and the potential charge of nanoparticles were dected. The enhanced photoacoustic and ultrasound imaging capability of nanoparticles in vivo were observed. Results The laser-induced phase -change liquid fluorocarbon nanoparticles were successfully prepared. The average particle size was 599.2 nm and the mean surface charge of was -24.1mv. The nanoparticles would undergo phase transformations with laser duration, meanwhile , the nanoparticles changed into microbubbles. The photoacoustic and ultrasound imaging capability of tumor were enhanced in contrast to control group. Conclusion The prepared laser-induced phase-shift nanoparticles could enhance the ultrasound and |
| Key words: ICG PFH Laser-induced phase-shift Ultrasound imaging, photoacoustic imaging |
