Objective To prepare a laser-induced phase -change liquid fluorocarbon nanoparticle and study on the phase-change in vitro and the enhanced photoacoustic and ultrasound imaging capability in vivo.Methods The nanoparticles with PLGA shell, PFH and ICG kernel were prepared by a three-step emulsification technique. The particle size and the potential charge of nanoparticles were dected. The enhanced photoacoustic and ultrasound imaging capability of nanoparticles in vivo were observed. Results The laser-induced phase -change liquid fluorocarbon nanoparticles were successfully prepared. The average particle size was 599.2 nm and the mean surface charge of was -24.1mv. The nanoparticles would undergo phase transformations with laser duration, meanwhile , the nanoparticles changed into microbubbles. The photoacoustic and ultrasound imaging capability of tumor were enhanced in contrast to control group. Conclusion The prepared laser-induced phase-shift nanoparticles could enhance the ultrasound and