Purpose: To explore the value of acoustic radiation force impulse (ARFI) imaging and CDFI in evaluating the effect of hepatic veno-occlusive disease (HVOD) after taking Gynura segetum.Materials and Methods: we prospectively measured stiffness of the liver and spleen with mean shear wave velocity (SWV, m/s) on ARFI imaging in 30 healthy volunteers and 29 patients of HVOD. Portal vein, right hepatic vein and splenic vein were evaluated by Doppler ultrasound, diameters and maximum velocities were measured and compared. We performed 5 shear-wave measurements in the liver and spleen, mean values were calculated. Results: B-mode sonography revealed patchy hypoechoic parenchymal areas (86%), thin hepatic veins (58.6%) and normal spleen (80%). The incidence of abnormal spectral in portal vein (89.6%) and right vein (58.6%) is high. A significant decline in the velocity of blood ?ow in portal veins , splenic vein and right hepatic veins were found in the HVOD cases as compared to those in the healthy controls. In addition, we observed a significant difference in diameter of hepatic vein between healthy subjects and patients with HVOD(P＜0.05). The mean liver and spleen stiffness value in healthy controls was significantly lower than in patients with HVOD（P＜0.001）. There was no significant differences in diameter of portal vein and splenic vein between healthy subjects and patients with HVOD（P>0.05）.Conclusion: CDFI can detect the change of blood vessel diameter and flow velocity, ARFI can detect the change of liver and spleen stiffness of patients. CDFI combined with ARFI have a higher value in clinical application for noninvasive diagnosis of HVOD.