| 摘要: |
| 目的 研究中性微泡(NMB)、阳离子微泡(CMB)及靶向阳离子微泡(CMB105)携内皮抑素质粒(ES)在超声作用下转染MDA-MB-231细胞裸鼠种植瘤,观察其对肿瘤模型生长的影响及治疗作用。
方法 双后肢皮下注射MDA-MB-231细胞悬液建立种植瘤模型，造模后14天，NMB、CMB、CMB105携ES质粒在超声（1 MHz, 2 W/cm2, and 50% DC, 30 seconds）作用下转染肿瘤，左侧肿瘤接受治疗，右侧肿瘤做为对照。治疗前及治疗后每3天观察记录肿瘤生长情况，绘制肿瘤生长曲线，治疗后15天处死动物，剥取肿瘤计算抑瘤率，行qPCR及Western-blot分别检测Endostatin mRNA及蛋白表达情况。
结果 通过绘制肿瘤生长曲线，在转染后12天开始，CMB治疗组及CMB105治疗组肿瘤生长均受到抑制，转染后15天，NMB、CMB及CMB105各治疗组抑瘤率分别为：10.63±1.47%、27.57±3.02%、53.18±5.69%，CMB105携带ES质粒在超声作用下成功抑制肿瘤生长。qPCR和Western-blot均检测到在CMB和CMB105组Endostatin mRNA及蛋白表达增加，两组间差异具有统计学意义（P＜0.01）。
结论 携载ES质粒的CMB105在超声作用下其抗肿瘤治疗效应明显优于CMB及NMB，可在一定程度上延缓肿瘤生长。 |
| 关键词: 超声 阳离子微泡 靶向 乳腺癌 内皮抑素。 |
| DOI： |
| 投稿时间：2016-03-21修订日期：2016-04-18 |
| 基金项目:包裹ICG的相变型阳离子分子探针靶向显像及治疗视网膜母细胞瘤的研究（No. 81501487） |
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| Study about the endostatin gene therapy effect on breast cancer in nude mice by ultrasound-targeted microbubbles destruction (UTMD) with netural microbubbles, cationic microbubbles and targeted cationic microbubbles.Zhou Yu, Xu Yan, Jian Jia |
| zhouyu,xuyan |
| () |
| Abstract: |
| Objective Study about the therapy effects on breast cancer in nude mice by UTMD with NMB, CMB and CMB105 carrying ES plasmid. Methods For inoculation, MDA-MB-231 tumor cell suspensions were injected subcutaneously in both hind limbs of nude mice. Xenograft tumors were allowed to grow for 14 days before the experimental studies began, NMB, CMB and CMB105 were injected into the tail vein, and 10 min after injection, ultrasound-mediated gene transfer was performed. Ultrasound was applied at 1 MHz, 2 W/cm2, and 50% duty cycle (DC) for 30 seconds, tumor xenografts on the left hind limb received the ultrasound mediated gene therapy and the right hind limb tumor xenografts were used as control. The size of tumor xenografts was recorded every 3 days. Fifteen days after transfer, the mice were sacrificed, the tumor growth inhibition rate was calculated and the tumor growth curve was delineated. qPCR and Western-blot were used to evaluate the level of ES mRNA and protein. Results UTMD-mediated endostatin gene delivery both with CMB and CMB105 significantly inhibited tumor growth, The inhibition rate of the CMB105 group was highest (53.18±5.69%) compared with CMB (27.57±3.02%) and NMB (10.63±1.47%). UTMD-mediated endostatin gene delivery with CMB105 could inhibit the tumor growth successfully. The level of ES mRNA and protein were increased both in CMB and CMB105 therapy group, and the difference between then was significant (P＜0.01). Conclusions UTMD-mediated ES gene delivery with CMB105 could inhibit the tumor growth successfully, the therapy effects were better than with CMB and NMB. |
| Key words: Ultrasound Cationic microbubbles Target Breast cancer Endostatin |
