Objective: To investigate the value of Spatio-temporal Image Correlation(STIC) technique in evaluating the left fetal ventrlenlar volume and systolic function in pregnant women with gestational diabetes mellitus(GDM).Methods：Fetal heart Ventricular volumes were measured using STIC technique in 46 women with GDM(GDM group)and 120 women with normal pregnancy(normal group).The virtual organ computer—aided analysis(VOCAL)technique was used to obtain left systolic and diastolic ventricular volumes．The stroke volume(LVSV),ejection fraction (LVEF)and cardiac output(LVCO) were calculated．Patients with gestational diabetes mellitus were divided into 2 groups：GDMI group: The plasma levels of FPG is less than 5.8 mmol/L, PPG is less than 6.7mmol／L; GDMII group: The plasma levels of FPG is higher than 5.8 mmol/L, PPG is higher than 6.7mmol/L. Results：Compared With normal,the left systolic and diastolic ventricular volumes of GDMI group was decreased (P<0.05); the left systolic and diastolic ventricular volumes and the left stroke volume(LVSV),ejection fraction(LVEF)and cardiac output(LVCO)of GDMII group was higher(P<0.05); Compared With GDMI group, the left systolic and diastolic ventricular volumes and the left stroke volume(LVSV),ejection fraction(LVEF)and cardiac output(LVCO)of GDMII group was higher(P<0.05); Conclusions: STIC is an adoptable reliable and noninvasive investigation of left fetal ventricular volume in pregnant women with gestational diabetes mellitus ,then can evaluate the systolic function.