Objective To explore the effect of patent ductus ateriosus on cerebral blood flow infusion in preterm infants. Methods: The research focus on the premature infants whose gestational period less than or equal to 35 weeks, birth weight less than or equal to 2200 gram and diagnosed with PDA born within 3 days. The experimental subjects are divided into two groups, the group of patent ductus arteriosus (PDA group), and the ductus ateriosus were closed (the control group). Using high-frequency color Doppler ultrasound with E-flow imaging to test the blood flow of MAC, LSA and tLSA of 62 cases (30 in PDA group, 32 in control group). The parameters including Vs, Vd, Vm, (S/D) and RI. Conducting a t-test to compare the result of the PDA group and the control group. Result: The statistics result of the gestational period and birth weight of the samples is not significant. The value of Vd of MCA, LSA and tLSA in the PDA group is significantly lower than the control group (p-value<0.001). The value of S/D and RI of MCA, LSA and tLSA in PDA group is significantly higher than the control group (p-value<0.001). There is no significantly difference of MCA, LSA’s Vs value between the two groups. The Vs of tLSA in the PDA group is significantly lower than the control group (p <0.001). Conclusion: The change of cerebral hemodynamic in premature infants with PDA was found by high-frequency ultrasound with E-flow color Doppler. It will be provide the basis to active intervention PDA, stabilize cerebral blood flow in the premature infants , avoid cerebral injury.