Objective To study the differences of transverse shear wave velocity(SWV) in all kinds of diffuse thyroid disease. Methods According to the clinical diagnosis, the research objects were divided into five groups: toxic diffuse goiter group (Graves disease) (group A, 45 cases), adolescence goiter group (group B, 30 cases), hypothyroidism group (C group, 42 cases), Hashimoto"s thyroiditis group (group D, 43 cases), subacute thyroiditis group (group E, 30 cases). SWV was measured by virtual touch tissue quantification(VTQ),then the data was statistically analyzed. Results SWV was (2.31±0.42)m/s in A group; (1.72±0.39) m/s in B group; (2.86±0.80)in C group; (2.64±0.54)m/s in D group; (4.48±2.01)m/s in E group; (1.89±0.38)m/s in F group. There was no significant difference between F group and B group(P＞0.05). The SWV in F group was statistical difference vs. other groups(P＜0.01).The SWV showed no significant difference between A and B group(P＞0.05), but there was statistical difference between A and other groups(P＜0.05).No significant difference of SWV was observed between C and D group(P＞0.05), but it was statistical difference between C and other groups(P＜0.01). There were no significant differences of SWV between D and A group, D and C group(P＞0.05).There was statistical difference between D and other groups(P＜0.01).There was statistical difference between E and each group(P＜0.01).Conclusions SWV was difference among the various types of diffuse thyroid diseases. VTQ has high clinical value for the diagnosis and differential diagnosis of diffuse thyroid disease.