Objective: To observe the right ventricular myocardial strain characteristics in patients with obstructive sleep apnea syndrome, to explore their clinical significance and clinical significance of the QLAB and velocity vector imaging (VVI) in the right ventricular systolic function measurement in patients with obstructive sleep apnea syndrome. Methods: 45 patients with obstructive sleep apnea syndrome were enrolled, got the right ventricular each segment myocardial strain curve in apex four-chamber view by QLAB and VVI software, recorded the right ventricle overall longitudinal systolic strain (GLSRV QLAB, GLSRV VVI).Results: GLSRV QLAB and GLSRV VVI showed a significant correlation with traditional echocardiography right ventricular systolic function parameters. GLSRV QLAB and RVFAC were correlated (r = 0.832, P < 0.001), GLSRV QLAB and TAPSE were correlated (r = 0.868, P < 0.001), GLSRV VVI and RVFAC were correlated (r = 0.716, P < 0.001), GLSRV VVI and TAPSE were correlated (r = 0.804, P < 0.001). GLSRV QLAB and GLSRV VVI were correlated (r = 0.833, P < 0.001).Determined right ventricular systolic dysfunction by RVFAC < 35% , the best cutoff value of GLSRV - QLAB for the detection of right ventricular dysfunction was -19.852% (95% confidence interval (CI): 0.735-0.951) (AUC = 0.869, P < 0.001), with a sensitivity of 80.0% and a specificity of 86.7% . the best cutoff value of GLSRV - VVI for the detection of right ventricular dysfunction was -19.99% (95% confidence interval (CI): 0.711-0.938) (AUC = 0.849, P < 0.001), with a sensitivity of 73.3% and a specificity of 86.7%.There was no significant difference in the comparison of AUC’s by the Hanley-McNeil method (difference = 0.020, 95% CI: 0.020 0.119, P = 0.693). Conclusion: GLSRV VVI and GLSRV QLAB could evaluate the right ventricular function in patients with obstructive sleep apnea syndrome, and could provide a new reliable approach for the assessment of right ventricular function.