| 摘要: |
| 目 的 探讨低频低强度超声对白色念珠菌的急性损伤情况,以及对其细胞壁通透性的影响。 方 法 配置浓度为1.5×107 cfu /ml的白色念珠菌菌液,取5ml菌液于一次性六孔培养板中。用频率为42KHz、探头直径为5cm的圆形平面超声治疗头,分别选择超声强度0.13W/cm2、0.35W/cm2辐照六孔板中的白色念珠菌菌液5min后,通过透射电镜和扫描电镜观察细菌外部形态、内部结构的损伤情况,并比较超声辐照后菌落在培养皿上的存活数。结 果 低频低强度超声辐照白色念珠菌后,扫描电镜下可见菌体明显肿胀、外形变大,细胞外液进入细胞内明显增加；透射电镜下见核物质在边缘聚集,细胞膜受损,大量水份进入细胞,形成空泡；不同剂量超声辐照48h后培养皿菌落计数显示,超声对白色念珠球菌的损伤有逆转作用。结 论 低强度超声辐照白色念珠菌能促进其细胞壁的通透性增加。 |
| 关键词: 白色念珠球菌 低频低强度超声 扫描电镜 透射电镜 细胞膜通透性。 |
| DOI： |
| 投稿时间：2016-06-21修订日期：2016-07-19 |
| 基金项目:重庆医科大学2015年国家自然科学基金预研资助项目(NSFYY201528) |
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| Study on the acute injury effect on Candida albicans by low-Frequency and low-intensity ultrasound |
| XIANG Yang |
| (College of Biomedical Engineering,Chongqing Medical University) |
| Abstract: |
| ABSTRACT Objective To investigate the effects of ?Low-Frequency?and??Low-Intensity
Ultrasound (LFLIU) on the acute injury of Candida albicans, and to investigate the effect of LFLIU on the permeability of the cell wall.Methods Concentration is 1.5 x 107 CFU / ml of Candida albicans bacteria liquid, take 5 ml of bacteria to one-off 6-well culture plate. With the frequency for 42KHz, probe diameter 5 cm circular planar ultrasonic treatment head, ultrasonic intensity 0.13W/cm2, 0.35W/cm2 irradiation 6-well plates Candida albicans bacteria solution for 5 min after, by transmission electron microscope and scanning electron microscope observation of bacterial morphology, internal structure damage, and to compare the ultrasonic irradiation after colony in the culture dish. The number of survival. Results LFLIU Candida albicans, scanning electron microscope (SEM) visible thalli were swollen shape becomes large, the extracellular fluid into the cells increased obviously; transmission electron microscope (TEM) see nuclear matter at the edge of the aggregation, cell membrane damaged, large amounts of water into cells, vacuolization; not with dosage ultrasound irradiation after 48h culture dish colony counting display, the damage of ultrasound on Candida albicans a reversal effect. Conclusion LFLIU irradiation can promote the increase of the permeability of the cell wall of Candida albicans. |
| Key words: Candida albicans LFLIU Scanning electron microscope Transmission electron microscope Cell membrane permeability. |
