ABSTRACT Objective To investigate the effects of ?Low-Frequency?and??Low-Intensity Ultrasound (LFLIU) on the acute injury of Candida albicans, and to investigate the effect of LFLIU on the permeability of the cell wall.Methods Concentration is 1.5 x 107 CFU / ml of Candida albicans bacteria liquid, take 5 ml of bacteria to one-off 6-well culture plate. With the frequency for 42KHz, probe diameter 5 cm circular planar ultrasonic treatment head, ultrasonic intensity 0.13W/cm2, 0.35W/cm2 irradiation 6-well plates Candida albicans bacteria solution for 5 min after, by transmission electron microscope and scanning electron microscope observation of bacterial morphology, internal structure damage, and to compare the ultrasonic irradiation after colony in the culture dish. The number of survival. Results LFLIU Candida albicans, scanning electron microscope (SEM) visible thalli were swollen shape becomes large, the extracellular fluid into the cells increased obviously; transmission electron microscope (TEM) see nuclear matter at the edge of the aggregation, cell membrane damaged, large amounts of water into cells, vacuolization; not with dosage ultrasound irradiation after 48h culture dish colony counting display, the damage of ultrasound on Candida albicans a reversal effect. Conclusion LFLIU irradiation can promote the increase of the permeability of the cell wall of Candida albicans.