The diagnosis and treatment of gynecological diseases has been growingly concerned, transvaginal ultrasonography in the diagnosis and treatment play a role, but it has its limitations. Three-dimensional ultrasound (3D-US) technology has developed and updated in recent years, widely used in the field of gynecology, including aspects of the uterus, uterine, accessories, fallopian tubes and assisted reproductive technology, scientific and accurate clinical diagnosis increasingly popular. This article will talk about the application in gynecology.