Objective: to value the imaging ability of high frequency ultrasonography for anatomic structure of vocal cord area. Methods: Ultrasonography was performed in 104 healthy volunteers. The anterior cross sections of vocal cord area were observed from front to back, taking thyroid cartilage as the acoustic window, and their real-time movements were also observed. The ultrasonographic features were summarized and the distribution of ultrasonic imaging levels in gender and its difference in age were analyzed, respectively. Results: The imaging ability of ultrasonography for vocal cord was manifested as the imaging depth. Detection rates of vocal cord structures were 100% in thyroid cartilage, 93.97% in false vocal cord, true vocal cord and arytenoid cartilage, 84.62% for rima glottidis and 73.08% for arytenoid muscle, posterior lamina of cricoid cartilage, respectively. There were significant differences of ultrasonic imaging levels in gender and of ages between different levels (P ＜ 0.01 in both). Conclusion: There was a good imaging ability of ultrasonogrphy for anatomic structures of vocal cord area in normal healthy adults, which can meet the need of study on ultrasonography’s evaluating vocal cord paralysis.