Objective To prepare loading recombinant tissue type plasminogen activator (rtPA) Pluronic -F127 (NP-rtPA) delivery system, and test the ultrasound effect in vitro and in rat hematoma model of cerebral hemorrhage in vivo, in order to evaluate its neurological behavior changes. Methods We prepared a NP-rtPA delivery system，assess the use of ultrasounds（US）to dissolve hematoma in vitro using as specimens from patients with hematoma hematoma in hypertensive cerebral hemorrhage, divided into control group, rtPA group, rtPA group, ultrasound NP-rtPA group and NP-rtPA US group, respectively, in the dissolution 6 h, 24 h, 72 h detection of hematomaand in a rat model of intracerebral hemorrhage （ICH）in vivo，which divided into rtPA, rtPA US, NP-rtPAand NP-rtPA US group;and evaluate the neurological response of the ICH rat model.The hematoma dissolution in vitro was measured at 6，24，and 72 hours. Then，to create a rat model of ICH in vivo，rtPA and NP-rtPA were injected into the hematoma cavity with US-controlled release of rtPA at scheduled times. The neurological behaviors of rats were valued in 0d, 1d, 3d, 7d,14d,21d and 28d with modified neurologic severity score (mNSS). Results NP-rtPA US dissolved hematoma 40% at 6 hours and 60% at 72 hours compared with the control rtPA （dissolving 20% and 40%，respectively）. There was an obvious difference at 0 and 1 day between the rtPA and Np-rtPA. The water content in brain tissue was found to be statistically different. Differences in the behavior of rats treated with rtPA US and Np-rtPA US were statistically significant at 21 and 28 days. Conclusion Ultrasound can control the release of rtPA from rtPA-Pluronic F127(NP-rtPA)，making it better at dissolving hematoma，which effect was better than that that achieved with common rtPA. In addition，this treatment may reduce brain edema and provide an effective method for the removal of residual hematoma in ICH using minimally invasive surgery.