Objective To investigate the application value for time intensity curve(TIC)in contrast enhanced ultrasound(CEUS)in the differential diagnosis of thyroid nodules(TNs)．Methods 163 patients with 167 TNs (62 papillary thyroid carcinomas, 30 nodular goiters, 6 Hashimoto thyroiditis and 30 adenomas) that underwent contrast-enhanced sonography with a linear probe (6-9 MHz, LOGIQE9/GE) were included in this study. Time intensity curve of TNs and the adjacent thyroid parenchyma were acquired with dedicated software.The shape and various parameters were recorded including peak intensity (Peak), time to peak (TtoPk), the area under the curve (Area), etc. Then calculate the different parameter values between TNs and the adjacent thyroid parenchyma such as △Peak, △Area and so on.The lesions were divided into group 1(≤1)and group 2(>1 cm)by maximum diameter.The shape of TIC and quantitative analysis findings of CEUS of nodules were compared.Results Benign and malignant TNs show different in wash-in and wash-out phase (P＜0.001) . Nodular goiters have identical-in and identical-out. Adenomas have earlyh-in and early-out. Papillary thyroid microcarcinomas have slow-in and early-out.While papillary thyroid carcinomas (>1 cm) have early-in and early-out. Peak and Area in benign TNs were higher than in maligant ones among group 1.(P＜0.05) Area, Peak and△Peak in benign TNs were higher than in maligant ones among group 2.(P＜0.05)Conclusion CEUS in combination with TIC analysis allows an evaluation of the microvascularisation of TNs and is helpful for the differentiation of benign and malignan nodules.