| 摘要: |
| 目的 制备一种热敏型纳米粒----包裹液态全氟己烷（PFH）的脂质纳米乳，探讨其术中评估射频消融边界的可行性。方法 采用均质-乳匀法制备包裹PFH的纳米粒。马尔文激光粒度分析仪检测纳米粒粒径范围，水囊实验观察纳米粒热相变情况。30只离体猪肝分别给予单纯射频消融、射频消融联合纳米粒处理，术中观察气化区的形成，并测量气化区及大体病理的大小，分析其相关性。结果 制备了平均粒径为（328±65.57）nm的热敏型纳米粒；体外加热实验示，当温度达到70℃时，二维超声观察纳米粒回声较加热前明显增强。离体猪肝实验示，加入纳米粒后射频消融产生的气化区更明显，而且与大体病理所示坏死范围呈高度相关。结论 通过均质-乳匀法成功制备了纳米粒，其粒径小且均一、具有热敏相变潜能，有望成为术中实时监控、评估射频消融边界的新方法。 |
| 关键词: 热敏 纳米粒 射频消融 离体猪肝 |
| DOI： |
| 投稿时间：2016-08-11修订日期：2016-10-23 |
| 基金项目:国家自然科学基金重点项目（No.81530056） |
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| Preparation of thermal-sensitive nanoparticle & an experimental study on radiofrequency ablation margins intraoperative assessment |
| jili,xiemingxing |
| (Union Hospital affiliated to Tongji Medical College Huazhong University of Science and Technology) |
| Abstract: |
| Objective To prepare a new type of thermal-sensitive nanoparticles(Nps), which is a kind of lipid nanoemulsion incorporated with liquid perfluorohexane（PFH）, and to investigate its feasibility in assessing radio-frequency ablation margins during operation. Methods Homogenization/emulsion method will be applied to prepare the nanoparticles that surround the PFH, and Malvin laser particle size analyzer will be applied to measure the diameter’s range of nanoparticles, as well as water sac experiment to investigate its thermal phase transition condition. Specifically, take 30pcs of pork liver for radio-frequency ablation(RFA) experiment with or without nanoparticles, then check the whole process of gasification zone formation and measure it, so does the general pathology. So that to analysis their correlation. Results The thermal-sensitive nanoparticles with a mean particle diameter（328±65.57）nm is successfully prepared. In in-vitro heating experiment we find, when the heating temperature reach 70℃, the echo of microsphere becomes much louder than pre-heating period through two-dimensional ultrasonography. And in-vitro liver experiment, the gasification zone formed by radio-frequency ablation becomes more obvious when adding in nanoparticle. Besides, that presents a high correlation with general pathology’s necrosis range. Conclusion The nanoparticles preparation by means of homogenization/emulsion technique is of slight and uniform particle diameter, with a potential of thermal-sensitive phase transition. So this preparation method can be regarded successful and has the prospect to come into being the new technique to realize real-time intraoperative monitoring, as well as a new way in assessing radio-frequency ablation margins. |
| Key words: Thermal-sensitive Nanoparticles Radio-frequency ablation Vitro porcine liver |
