Objective To prepare a new type of thermal-sensitive nanoparticles(Nps), which is a kind of lipid nanoemulsion incorporated with liquid perfluorohexane（PFH）, and to investigate its feasibility in assessing radio-frequency ablation margins during operation. Methods Homogenization/emulsion method will be applied to prepare the nanoparticles that surround the PFH, and Malvin laser particle size analyzer will be applied to measure the diameter’s range of nanoparticles, as well as water sac experiment to investigate its thermal phase transition condition. Specifically, take 30pcs of pork liver for radio-frequency ablation(RFA) experiment with or without nanoparticles, then check the whole process of gasification zone formation and measure it, so does the general pathology. So that to analysis their correlation. Results The thermal-sensitive nanoparticles with a mean particle diameter（328±65.57）nm is successfully prepared. In in-vitro heating experiment we find, when the heating temperature reach 70℃, the echo of microsphere becomes much louder than pre-heating period through two-dimensional ultrasonography. And in-vitro liver experiment, the gasification zone formed by radio-frequency ablation becomes more obvious when adding in nanoparticle. Besides, that presents a high correlation with general pathology’s necrosis range. Conclusion The nanoparticles preparation by means of homogenization/emulsion technique is of slight and uniform particle diameter, with a potential of thermal-sensitive phase transition. So this preparation method can be regarded successful and has the prospect to come into being the new technique to realize real-time intraoperative monitoring, as well as a new way in assessing radio-frequency ablation margins.