【】Objective To evaluate right ventricular systolic function in patients with pulmonary hypertension(PH) using real-time three-dimensional echocardiography. Methods a total of 60 patients with PH Were enrolled in this study, they were divided into three groups according to pulmonary arterial pressure (PASP) level：mild group and moderate group and severe group, each group consisted of 20 cases, 20 healthy people were enrolled As controls. Firstly, tricuspid annular plane systolic excursion(TAPSE) were measured by M-mode, right ventricular fractional area of change(RVFAC) were measured by 2-DE; Secondly, tissue Doppler-derived tricuspid lateral annular systolic velocity(S’) and calculated right ventricular index of myocardial lperformance(RIMP); In the end, right ventricular end-diastolic volume(RVEDV) and right ventricular end-systolic volume (RVESV) were measured by Real-time Three-dimensional Echocardiography(RT-3DE) and calculated right ventricular stroke volume (RVSV) and right ventricular ejection fraction (RVEF). Results There are no differences of RVEDV among every group(P>0.05), the RVESVs were higher while RVSVs and RVEFs were lower with the increase of the degree of pulmonary arterial hypertention, but it has no different between moderate and severe groups(P>0.05); A positive correlation was found between RVEF and RVFAC、s’、and TAPSE(r=0.658, p=0.000; r=0.832, p=0.000; r=0.886, p=0.000, respectively ), there was a negative correlation between RVEF and RIMP(r=-0.759, p=0.000). Conclusions Real-time three-dimensional echocardiography can assess right ventricular systolic function in patients with PH accurately and provide a guidance for clinical evaluation and prognosis judge.