| 摘要: |
| 目的 制备一种新型的具备特异性肝脏靶向性能的超声造影剂—携半乳糖化多聚赖氨酸的脂质包裹的纳米级液态氟碳微球超声造影剂，并研究其在体外的肝脏寻靶能力。方法 应用还原胺化法优化制备肝脏去唾液酸糖蛋白受体的配体——半乳糖化多聚赖氨酸（Gal-PLL），用其将制备成功的脂质膜洗脱下来并逐滴加入液态氟碳，通过超声波细胞粉碎机进行振荡获得目标造影剂，并观察其形貌、检测其粒径及电位。将靶向造影剂（实验组）与非靶向造影剂（对照组）同时滴入贴壁生长的正常大鼠肝细胞（BRL）及正常人肝细胞（L02）中，比较观察两组造影剂与细胞的靶向效果。结果 成功制备出肝脏去唾液酸糖蛋白受体的配体Gal-PLL，并合成了靶向纳米液态氟碳脂质微球超声造影剂，电镜下其形态圆整、大小均一，性质稳定,其平均粒径在200 nm左右，平均电位在﹢35mV左右。制备成的靶向纳米液态氟碳脂质微球超声造影剂能靶向聚集于体外BRL及L02细胞膜周围。结论 自制携的脂质包裹的纳米液态氟碳微球超声造影剂具有性质稳定、靶向性等优点，为今后实时在体监测并定量评估肝脏损伤程度的超声显影研究提供前期基础。 |
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| DOI： |
| 投稿时间：2016-11-01修订日期：2017-04-06 |
| 基金项目:国家自然科学基金青年基金(31300688) |
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| The Manufacture of a Novel Ultrasound Contrast Agent: Liver Targeted Perfluorocarbon Lipid Nanoparticle, and Its Targeting Study in Vitro of Liver |
| Shi Hong,Yu Jinhong |
| (Department of Function,the first people’s hospital of Neijiang) |
| Abstract: |
| Objective To manufacture a new-type ultrasound contrast agent which had the specificity of liver targeting—-- perfluorooctyl bromide lipid nanoparticle with Galactocylated Poly-L-Lysine（Gal-PLL），to study its liver targeting ability in vitro. Methods The preparation of high-efficiency Galactocylated Poly-L-Lysine（Gal-PLL）ligand of the asialoglycoprotein receptor in liver was optimized by Chemical reactions of reductive amination. Lipid membrane prepared before was eluted with Gal-PLL，adding PFOB in it， then objective ultrasound contrast agent was manufactured by means of ultrasonic cell disruptor agitation，observing its feature，detecting the grain diameter and electric potential. Targeting contrast agent（experimental group） and non-targeting contrast agent（control group） were instilled into normal rat hepatocyte BRL and normal human hepatocyte L02 which grew with adherence，observing the two groups’targeting effect of the contrast agent and the cell. Results High-efficiency Gal-PLL ligand of the asialoglycoprotein receptor in liver was successfully prepared and novel ultrasound contrast agent—--perfluorooctyl bromide lipid nanoparticle was manufactured. Its shape under the electron microscope was rounded and the same size ，it had stable nature also. Its average diameter was 200 nm, and its average electric potential was 35 mV. The contrast agent can flock together around the BRL and LO2 cells because of its targeting effect. Conclusion Manufactured novel ultrasound contrast agent—--perfluorooctyl bromide lipid nanoparticle had many advantages，such as targeting and stable nature. These advantages provided a primary foundation for future study of ultrasonic contrast of real time monitoring in vivo and quantitative assessment of the liver damage degree. |
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