Objective To assess the changes of left and right ventricular diastolic hemodynamics in patients with atrial septal defect by enhanced Vector flow mapping. Methods 26 adult ASD patients and 31 control subjects were enrolled in this study. The conventional parameters and VFM parameters: The Left and right ventricular diastolic peak velocity、diastolic flow、diastolic total flow and vorticity in apex-mid-basis segment were analyzed by VFM technique. Results The left ventricular diastolic IQ of basal and apical segment、TQ of basal segment were lower（P<0.05）than those of the control group, while right ventricular diastolic Vd、IQ and TQ of all segments were higher(P<0.05) compared with the control group. The left ventricular vorticity of middle and apical segment and right ventricular vorticity of all segments are higher（P<0.05）than those in control group．Conclusions The positive blood flow and vorticity of the left and right ventricle are different between adult ASD patients and normal subjects. Vector flow mapping is an ideal and noninvasive method to evaluate the hemodynamics of cardiac chambers.