Objective: To explore the clinical application value of contrast-enhanced ultrasound (CEU) in the diagnosis of tubal infertility(TIF). Methods: 335 fallopian tubes from 176 subjects diagnosed as infertility. All of them were diagnosed by CEU. Laparoscopic hydrotubation (LH) were also performed as a standard diagnosis, and then compared with the results of CEU diagnosis. Results: Compared with LH, the sensitivity, specificity and accuracy values of CEU in the diagnosis of fallopian tubes obstruction were 92.2% (237/257), 82.1%(64/78) and 89.9% (301/335) respectively. The results of CEU showed that 78 fallopian tubes from 40 subjects (22.7%, 40/176) were unobstructed and 257 fallopian tubes from 136 patients (77.3%, 136/176) were obstructed. For unobstructed subjects, the fallopian tubes were normal, soft and hydrosalpinx. Contrast agents showed even dispersion after injected into the pelvic cavity through both fallopian tubes. For obstructed subjects, fallopian tubes were discontinuity and irregularity, and few or no contrast agents, when injected the contrast agents through both fallopian tubes. No or uneven overflow of contrast agents were found dispersion in the pelvic cavity. The results of LH showed that 84 fallopian tubes from 43 subjects (24.4%, 43/176) were unobstructed and 251 fallopian tubes from 133 patients (75.6%, 133/176) were obstructed. In addition, the results of LH also showed a large amount of injection overflow was found in unobstructed patients, while little or no injection overflow was found in obstructed patients. Conclusions: CEU has high values of sensitivity, specificity and accuracy in the diagnosis of TIF, which plays an important role in diagnosis for patients suffering from TIF, and thus deserves to apply to the clinic.