【Abstract】Objective To evaluate global strain of left ventricular in acute myocardial infarction canine using two-dimensional speckle tracking imaging. Methods The study included a total of 27 healthy adult canines. AMI canines were induced by ligation of left anterior descending coronary artery. Toshiba Artida color Doppler diagnostic instrument was used to collect and store standard and parasternal axis views of left ventricular images. And 2DT(two-dimensional speckle tracking, 2DT) online analysis software was used to analyze the global LS strain. Results LVEVS（left ventricular end-systolic volume， LVEVS） increased and LVEF（left ventricular ejection fraction，LVEF） decreased significantly compared with the preoperative. LS（longitudinal strain,LS) of septum and lateral wall in middle level, anterior wall and every segments in apex level were decreased after AMI compared with those before AMI. Conclusions Using two-dimensional speckle tracking imaging can find deformation ability reduced after myocardial infarction. 【Key Words】2DT; canine; strain