ABSTRACT Objective Stroke volume index (SVi) has been proposed as potential value in patients with aortic stenosis (AS). The aim of this study was to evaluate the relationship between SVi measured by three dimensional echocardiography and left ventricular (LV) global longitudinal strain in patients with AS. Methods Fifty-two patients with AS underwent echocardiographic examination, including three dimensional echocardiography and two-dimensional speckle-tracking strain measurements. Patients were retrospectively grouped according to SVi (<35ml/m2) into normal stroke volume groups (n=33) and low stroke volume groups (n=19). It were calculated to LV global longitudinal strain (GLS), basal longitudinal strain (BLS), medial longitudinal strain (MLS) and apical longitudinal strain (ALS) with two-dimensional speckle-tracking echocardiography. Results There was significantly different between two groups in left ventricular ejection fraction (LVEF, 60.9±3.0 vs 49.4±6.8，P<0.001), GLS ( -18.7±2.7 vs -13.3±2.9，P<0.001), BLS（-16.2±3.4 vs -9.8±5.6，P<0.001）、MLS（-18.7±3.2 vs -12.7±5.7，P<0.001）、ALS（-22.9±4.9 vs -19.6±6.8，P<0.045） and peak transvalvular pressure gradient (PGmax, 68.6±19.8 vs 40.1±10.3，P<0.001）. SVi showed the great correlations with the outcome of LVEF（r=0.788，R2=0.621，P<0.001）and GLS（r=0.799，R2=0.639，P<0.001）. There were the correlations between SVi and aortic valve area (r=0.204，R2=0.042，P=0.147). Conclusion Among the echocardiographic measures, stroke volume index (SVi) has the strongest association with the great correlations with left ventricular global longitudinal strain and ejection fraction.