Objective To evaluate the strain rate of left ventricular segment myocardium in different diastolic function by Speckle Tracking Imaging(STI), and to compare the relationship between segment diastolic function and integral diastolic function. Methods Mitral valve flow E peak and A peak were measured by Doppler, mitral annulus eSp peak in ventricular septum and eL peak in lateral wall were acquired. All patients were divided to three groups: Group A: Left ventricular diastolic function is normal; Group B: Left ventricular compliance is reduced; Group C: Left ventricular diastolic function is pseudo normalization. To analyze the early/late strain rate(ESR and ASR) of myocardial relaxation in each segment by STI. Results With the decrease of integral diastolic function, the ESR in each segment also was decreased, but the ASR in each segment was increased. Analyse the change of ESR and ASR in three groups, the amplitude of the middle segments and apical segments is larger than that of the basal segments. Within group B and group C, the maximal change of ESR and ASR is apical myocardium. Conclusions STI is helpful for early detection of diastolic dysfunction in segmental myocardium; With diastolic dysfunction, apical ESR continued to decrease, ASR increase first and then decrease.