Objectives The aim of this study was to explore the value of layer-specific strain on assessing the early damage of the left ventricular (LV) systolic function in asymptomatic and left ventricular ejection fraction (LVEF) well-preserved patients with late-onset severe preeclampsia (LO-SPE) . Methods A total of 51 patients with LO-SPE(LO-SPE group), 54 age-,gestational age-matched normal pregnancy woman(NP group) and 50 age-matched non-pregnant women as control group were included. Longitudinal strains of the endocardium, myocardium, and epicardium (GLSendo, GLSmyo, and GLSepi ) were obtained by a software enabling the analysis of strains in three myocardial layers.Date from women in late pregnancy and 3 months after delivery.Results GLSendo, GLSmyo, and GLSepi were significantly reduce in NP group compared with control group(P<0.005),and subsequently returned postpartum to values comparable to the control(P>0.005). GLSendo, GLSmyo, and GLSepi were significantly reduce in LO-SPE group compared with NP group and control, and returned to above baseline values after delivery(P<0.005), and all strain index except GLSendo returned to normal level comparable to the control (P<0.005). Conclusions Layer-specific evaluation of the left ventricle has great value in evaluating early impairment of LV in patients with LO-SPE. And this relatively novel technique may made it possible to help us identify patients at high-risk , thus early intervention to preventing further damage and ensure perinatal safety.