Objective To investigate the clinical value of ultrasound guided cut needle biopsy from external path of neck in the diagnosis of parapharyngeal space neoplasms. Methods 43 cases of parapharyngeal space neoplasms underwent ultrasound guided cut needle biopsy from external path of neck, and histology results were compared with surgical pathology. Results Among 43 cases, the consistency rate of biopsy results and surgical pathology was 97.7%(42/43). No major complication induced by biopsy was found. Conclusion With high accuracy and fewer postoperative complications, Ultrasound guided cut needle biopsy from external path of neck is simple, and safe, could be used in diagnosis of parapharyngeal space neoplasms.