Objective: To discuss the application of CEUS in quantitative evaluation renal cortical and medullary perfusion in acute renal failure in rabbits. Methods: Rabbit ARF models were established with intramuscular 50% glycerin (12-15 ml/kg) injection into 16 New Zealand rabbits" thighs and carried out CEUS examination before and 24h after injection and analyzed the perfusion time-intensity curve (TIC) by Q-LAB. The parameters including arrival time(AT), time to peak intensity(TTP), amplitude o f peak intensity(A), slope rate of TIC(β)and area under the curve(AUC). Results: the values of A and β of renal cortex before injection were (17.24±10.31dB)and (5.24±2.10dB /s), respectively, which was significantly higher than values after injection 24 hours later, i.e. (7.32±4.81dB)and(1.62±1.20dB /s)(P＜0.05). The values of TTP and AUC of renal cortex before injection were (2.48±1.81s)and(325.27±121.60dB s),respectively, which was significantly lower than values after injection 24 hours later, i.e. (6.10±3.41s)and(655.39±271.80dB s)(P＜0.05). Before renal failure in rabbits, the difference of AT, serum urea nitrogen (BUN)、serum creatinine (SCr)、resistance index(RI) and peak systolic velocity(PSV) after 24 hours were not significant. (P＞0.05)Conclusion: the Contrast-enhanced ultrasonography quantitative technology can effectively reflect condition of the cortical and medullary perfusion in acute renal failure in rabbits. It is more sensitive than biochemical indicators of serum urea nitrogen (BUN), serum creatinine (SCr). It facilitates the early diagnosis of acute renal failure and has certain application value.