Objective To study the change of elasticity in rabbits’ kidney in low temperature conditions. Methods 20 New Zealand rabbits were randomly divided into five groups，4 rabbits in each group .The first group was considered as control group which was under room temperature conditions .The second group was treated under-5℃~-14℃for 4hours.The third group was treated under-5℃~-14℃for 4hours.The fourth group was treated under-15℃~-24℃for 8hours.The fifth group was treated under-15℃~-24℃for 8hours．The echo of kidney were evaluated by two-dimensional ultrasound and shear wave velocity(SWV) were quantitatively analyzed by Acoustic Radiation Force Impulse (ARFI) technique after treatment . Results No significant changes were found in the echo of kidney in any group. Compared with the control group，the SWV of the other groups were significantly increased (P＜0.05). Compared with the second group，the SWV of the fourth and fifth group were significantly increased (P＜0.05). Compared with the third group，the SWV of the fourth and fifth group were significantly increased (P＜0.05). Compared with the fourth group，the SWV of the fifth group were significantly increased (P＜0.05).Conclusions Acoustic Radiation Force Impulse technique can reflect the change of elasticity in rabbits’ kidney in low temperature conditions and provide useful information for the early diagnosis of renal damage caused by low temperature for clinical research．