| 摘要: |
| 目 的 探讨低频低强度超声联合氢醌药膏对SD大鼠黄褐斑病灶治疗的作用,有望为临床治疗黄褐斑提供新的思路。方 法 将建模成功的18只SD大鼠黄褐斑模型随机分对照组、低强度超声联合氢醌组、氢醌组,每组6只。联合组用声强0.32W/cm2、频率为42KHz的超声辐照12min；氢醌组脱毛后均匀涂上氢醌轻揉至大概吸收；对照组则保持皮肤暴露,不做任何处理。1.实验过程中观察SD大鼠生理指标变化。2.采集斑块图片并观察变化趋势。3.采取血清、肝脏、皮肤,测其SOD活力和MDA含量。4.将皮肤制作病理切片,光镜下观察黑素颗粒的变化。 结 果 1.各组SD大鼠治疗前后各项生理指标无明显变化。2.超声联合氢醌组的斑块面积减少,颜色变浅；氢醌组变化略小。3.治疗后SOD活力均有上升,MDA含量均有下降,但是超声联合氢醌组更为明显,超声联合氢醌组有显著差异(P＜0.05)。4.病理可见治疗组黑色颗粒减少,超声联合氢醌组黑色素颗粒散在分布,氢醌组黑色素颗粒线性分布。 |
| 关键词: 大鼠 黄褐斑 低频低强度超声 氢醌 协同作用 |
| DOI： |
| 投稿时间：2017-05-31修订日期：2017-06-19 |
| 基金项目:重庆市基础科学与前沿技术研究专项(csct2016jcyjA0098) |
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| Experimental Study of Low-Frequency and Low-Intensity Ultrasound Joint hydroquinone Ointment to SD Rat Model of Chloasma |
| He shiyi,Du yonghong |
| (State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology,Chongqing Key Laboratory of Ultrasound in Medicine and Engineering,College of Biomedical Engineering,Chongqing Medical University) |
| Abstract: |
| Abstract : Objective: To investigate the treatment effect of low intensity ultrasound combined with hydroquinone ointment on chloasma of SD rats in order to provide new ideas for clinical treatment of chloasma. Methods: Eighteen SD rats were randomly divided into control group, low intensity ultrasound combined with hydroquinone group, and hydroquinone group, with 6 rats in each group. The combined group was irradiated with ultrasound at the intensity of 0.32W/cm2 and frequency of 12min (42KHz); the hydroquinone group was evenly coated with hydroquinone to absorb after the hair removal; while the control group only expose the skin without any treatment. The changes of physiological indexes in SD rats were observed during the experiment. Plaque images were collected and the change trend was observed. Serum, liver, and skin were removed, and their SOD activity and MDA content were measured. Pathological sections of skin were made, and the changes of melanin granules were observed under optical microscope. Results: 1. There were no significant changes in physiological indexes before and after treatment among three groups of SD rats. 2.Plaque area in ultrasound combined with hydroquinone group reduced and became pale, while hydroquinone group did not show obvious changes. 3.After treatment, the activity of SOD was increased, while the content of MDA was decreased, with the changes being more obvious in the ultrasound combined with hydroquinone group. There was significant difference between the ultrasound combined with hydroquinone group and the hydroquinone group (P < 0.05). 4.Pathological observation showed that the number of black particles were reduced in the treatment groups, and the distribution of melanin granules was discrete in the combined group and linear in hydroquinone group. Conclusions: Low intensity ultrasound combined with hydroquinone is significantly superior to single hydroquinone in its bactericidal effect on SD rats with chloasma. |
| Key words: Rat chloasma low frequency and low intensity ultrasound Hydroquinone Synergies |
