| 摘要: |
| 目的 探讨载自杀基因的靶向微泡联合超声对视网膜母细胞瘤（RB）的抑制作用。方法 制备携带单纯疱疹病毒Ⅰ型胸苷激酶（HSV1-tk）质粒和VEGFR2抗体的靶向微泡，分为空白对照组、细胞+质粒组、细胞+质粒+SonoVue组、细胞+靶向微泡组、细胞+质粒+超声辐照组、细胞+质粒+SonoVue+超声辐照组和细胞+靶向微泡+超声辐照组进行实验。荧光显微镜观察基因转染情况，流式细胞仪检测转染率，加入丙氧鸟苷（GCV）后，检测细胞的抑制率。结果 细胞+靶向微泡+超声辐照组的转染率为（24.78±1.04）%，较细胞+质粒+SonoVue+超声辐照组（14.31±0.69）%高。随着GCV浓度的增加，培养时间的延长，各组的抑制率逐渐升高。当GCV浓度在100 mg/l，培养96h后，细胞+靶向微泡+超声辐照组对RB细胞的抑制率达（92.91±1.71）%。结论 在加入GCV后，携带HSV1–tk的靶向微泡联合超声能有效的抑制RB细胞。 |
| 关键词: 超声靶向微泡破坏 靶向微泡 视网膜母细胞瘤 基因转染 |
| DOI： |
| 投稿时间：2017-06-01修订日期：2017-09-24 |
| 基金项目:国家自然科学基金项目； 上海市自然科学基金 |
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| Study of ultrasound targeted carry HSV1-tk targeted microbubble destruction inhibition retinoblastoma |
| wu ying,du lianfang |
| () |
| Abstract: |
| Objective To evaluate inhibitory effect of retinoblastoma（RB） by ultrasound targeted carry HSV1-tk targeted microbubble destruction. Methods Carring HSV1-tk and VEGFR2 antibody targeted microbubble was Prepared. All the RB cells were divided into 7 groups：control group，cells+plasmid，cells+plasmid+SonoVue，cells+targeted microbubble，cells+plasmid+ultrasound，cells+plasmid+SonoVue+ultrasound，cells+targeted microbubble+ultrasound. Gene transfection results were observed under inversion fluorescent microscope. Seventy–two hours later，gene transfection rates were guantified under flow cytometry. Inhibitory rate of RB cells were evaluated after GCV was added. Results The transfection rate of cells+targeted microbubble+ultrasound group was（24.78±1.04）%，which was higher than cells+plasmid+SonoVue+ultrasound group（14.31±0.69）%. With the increase of GCV concentration and the prolongation of culture time, the inhibition rate of each group increased gradually. When the GCV concentration was 100mg/l，afer ninety–six hours culture time， inhibitory rate of cells+targeted microbubble+ultrasound group were （92.91±1.71）%. Conclusion Ultrasound targeted carry HSV1-tk targeted microbubble destruction can effectively inhibit RB cells，after GCV was added. |
| Key words: Ultrasound-targeted microbubble destruction Targeted Microbubble Retinoblastoma Gene transfection |
