Objective To investigate the application value of TEE in cystic disease on cardiac valves during the perioperative period. Methods Retrospective analysis of TTE and / or TEE for the diagnosis of cystic disease on cardiac valves, The characteristics of ultrasound imaging were summarized, surgical and pathological results were used as a control. Result All of the 5 cases have characteristic ultrasonographic manifestations . In all cases, cystic disease on cardiac valves were detected by TEE, which were not detected in 2 cases by TTE. 3 cases were diagnosed as cyst on cardiac valves by the surgical and pathological, 2 cases were diagnosed as cystic disease on cardiac valves by the surgical. There was a good correlation between preoperative measurement size and intraoperative measurement size. sample correlation coefficient r=0.998,P<0.01. Conclusion TEE has important value in the positioning and qualitative diagnosis of cystic disease on cardiac valves.