ABSTRACT Object To investigate the blood perfusion enhancement in breast cancer induced by diagnostic ultrasound and microbubbles. Methods five breast cancer patients confirmed by pathology were treated using diagnostic ultrasound and microbubbles within an hour after neoadjuvant chemotherapy. Contrast-enhanced ultrasound(CEUS)before and after the treatment were captured respectively. Then quantitation analyzes of CEUS include peak intensity(PI), the area under cave(AUC) and ascending slope(AS). Results CEUS demonstrated that the tumor perfusion was enhanced, with a significant augment of PI, AS and AUC. There were significant differences of PI and AS before and after treatment(Z=-2.13,-2.09;P=0.03,0.03). But there were no obvious differences of AUC before and after treatment（t=-1.15;P=0.28）. Conclusion Microbubble mediated ultrasound may increase the blood perfusion of breast cancer.