Objective: To make sure that the insufficient of oocyte autocrine factor of growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) plays an important role in the incidence of premature ovarian failure induced by cyclophosphamide and further elucidate low intensity pulsed ultrasound could regulate the expression of oocyte autocrine cytokines to protect the ovaries.Methods:13 rats were randomly selected as normal group , and the other 50 rats were intraperitoneally injected with cyclophosphamide 30mg/kg for 5 days to establish the animal model of premature ovarian failure. A total of 43 rats were sucessfully established the animal model. Then the rats were randomly divided into control group (n=21) and treatment group (n=22).The treatment group was treated with low-intensity pulsed ultrasound, and the normal and control group were treated with the same method as the treatment group, but the instrument had no power output. Western blotting were used to detect the expression of GDF9 and BMP15 proteins. Resultts: After CTX injection, compared with the normal group, in the control group, the expression of GDF9 and BMP15 proteins were decreased significantly (P=0.01).After ultrasound treatment, compared with the control group, in the treatment group, the expression of GDF9 and BMP15 proteins were increased significantly (GDF9,P=0.01;BMP15,P=0.00). Conclusion：Low intensity pulsed ultrasound has a protective effect on ovarian. It can promote the expression of GDF9 and BMP15 protein, thus promote the follicular development.