Purpose: To evaluate the value of conventional ultrasound combined virtual touch tissue imaging quantification(VTIQ)in typeⅡdiabetes with diabetic peripheral neuropathy. Method:Forty-four patients with diabetic peripheral neuropathy(DPN group) and thirty healthy volunteers (normal control group) were retrospectively analyzed from December 2015 to January 2017.The ultrasonographic features of tibial nerves were observed. Besides, the cross-sectional area (CSA) and shear wave velocity (SWV) were measured. Results: The tibial nerves of DPN group were wider than those of normal control group, the echo was decreased, “cellular structure” was fuzzy, and epineurium became thicken. The value of CSA and SWV were increased compared with normal control group, the difference was statistical significance(P<0.05). Conclusion: Conventional ultrasound (US) can show the nerve structure and scope of lesions of patients with DPN. It can provide morphological information. VTIQ can reflect the hardness of the affected nerve, and provide functional information. The combination methods can further improve the diagnostic ability of DPN.