【】Objective To evaluate global and regional left ventricular(LV) strain of patients with chronic kidney disease(CKD) by three-dimensional speckle tracking imaging(3D-STI) and discuss the possible association between global strain, regional strain and ejection fraction of LV. Methods The study includes 48 CKD patients, including 21 patiients with EF≥55% in group A, 27 patients with EF＜55% in group B, and 20 healthy controls. Global peak longitudinal strain(GSL),global peak area strain(GSA), global peak radial strain(GSR), regional peak longitudinal strain(rSL), regional peak area strain(rSA), regional peak radial strain(rSR) were measured by 3D-STI. Then possible association between GSL, GSA, GSR and LVEF were discussed by the Pearson correlation analysis. Results ①Global peak strain: The GSL, GSA, GSR were statistically different in the control group, patients in group A and group B(p <0.01). There were significant differences between groups(p <0.01)as following, GSL was lower in group A(p <0.01), all of GSL, GSA, GSR were lower in group B compared with control group (p <0.01). ②Regional peak strain: The rSL, rSA, rSR were statistically different in the control group, group A and group B(p <0.05).Compared with control group, rSL of basal segments were lower in group A, while there were no differences in middle and apical segments. Compared with control group and group A, rSR, rSA in group B were lower from the basal to the apical segments(p <0.05). The Pearson correlation analysis revealed GSL, GSA, GSR were strongly associated with LVEF(r=-0.712、0.760、-0.810 p < 0.01).Conclusions 3D-STI is an effective way to evaluate the systolic strain of CKD patients. The global and regional peak longitudinal strain can recognize systolic dysfunction more sensitively.