Malignant tumors are a type of disease with high mortality, and chemotherapy is one of the important treatments. The application of microbubble sonoporation effect to increase the uptake of chemotherapeutic drugs by cells to increase the local drug concentration of tumor tissue to achieve the purpose of sensitizing chemotherapy is one of the new directions of tumor treatment research. Ultrasound combined with microbubbles can increase the local drug concentration of tumor tissue, enhance cytotoxicity, promote tumor cell apoptosis, reduce tumor volume, improve tumor resistance to chemotherapeutic drugs, and have good clinical application value and prospect. This paper is intended to summarize the basic research and clinical application of ultrasound combined with microbubbles sensitizing tumor chemotherapy.