| 摘要: |
| 目的 制备一种包裹ICG的靶向相变型阳离子脂质纳米粒(INP),连接CD105抗体,检测其光热效应、体外相变、光声及超声显像规律,并证实纳米粒抗体连接成功。方法 采用双乳化法制备包裹ICG及液态氟碳(PFP)的阳离子脂质纳米粒；链霉亲和素法连接CD105抗体,流式细胞量化及激光共聚焦下观察抗体连接情况;脉冲激光激发观察其光热效应及相变情况；体外Lifu辐照致相变后记录超声显影效果；光声仪检测光声显像能力。结果 制备的INP平均粒径(354.2±93.85nm),平均电位(25.2±3.29mv),与CD105抗体结合率为99.89%,纳米粒具有良好光热效应,一定浓度下2W/cm2激光辐照180s温度可升至63℃,可发生液气相变。体外超声及光声显影效果佳。结论 成功制备了包裹ICG的靶向相变型阳离子脂质纳米粒(INP),其光热效应好、光声显影及增强超声显影效果佳,纳米粒与CD105抗体结合率高。 |
| 关键词: 阳离子，脂质纳米粒，吲哚菁绿，相变 |
| DOI： |
| 投稿时间：2018-03-28修订日期：2018-04-03 |
| 基金项目:国家自然科学(No. 81170858;81501487) |
|
| The preparation of ICG -incoperated targeted cationic nanoparticles and imaging in vitro |
| zou hongmi,li meng,zhou xiyuan,jian jia |
| (Second Hospital Affiliated to Chongqing Medical University,Chongqing) |
| Abstract: |
| Objective To prepare a target phase variant cationic lipid nanoparticle (INP), which is encapsulated with ICG, to connect the CD105 antibody, to detect its photothermal effect, in vitro phase transition, photoacoustic and ultrasonic imaging, and to confirm the successful connection of the nanoparticle antibody. Methods The cationic lipid nanoparticles with ICG and liquid fluorine carbon (PFP) were prepared by double emulsification method, the linkage of CD105 antibody by streptomyces affinity, flow cell quantification and laser confocal observation of antibody connection; The photothermal effect and phase transition were observed after laser irradiation. After the phase change through Lifu irradiation in vitro, the ultrasound imaging was recorded. The photoacoustic imaging capability is detected by a photoacoustic instrument. Results The average particle size (354.2±93.85nm), average potential (25.2±3.29mv) and CD105 antibody binding rate were 99.89, the nanoparticles had good photothermal effect, and the 180s temperature of 2w/cm2 laser irradiation could rise to 63 ℃ at a certain concentration, the INP Liquid gas phase change can occur. In vitro ultrasound and photoacoustic imaging effect is good. Conclusion The target phase variant cationic lipid nanoparticles (INP) coated with ICG were successfully prepared, and the effect of light thermal effect, photoacoustic imaging and enhanced ultrasound was good, and the binding rate of nanoparticles and CD105 antibody was high. |
| Key words: Cationic, lipid nanoparticles, indocyanine green, phase change. |
