Objective To investigate the value of ultrasound preoperative Evaluation and precise location in Vacuum Assisted Minimally Invasive Rotary Cutting System for the treatment of breast multiple benign lumps. Methods 52 patients with multiple benign breast lumps (156 lesions) admitted to our hospital from January 2016 to October 2017 were retrospectively analyzed. Among them, 26 cases (78 lesions), who were performed by breast tumor rotation operation under ultrasound guidance after preoperative ultrasound evaluation and accurate location, were randomly selected as a study group; in addition, 26 cases (78 lesions) , who were performed by breast tumor rotation operation under ultrasound guidance after conventional ultrasound examination, were randomly selected as a control group. The operation time, the number of rotary cutting knives and the incidence of intraoperative hemorrhage were compared with two groups. Results All lumps were completely removed. The operation time (19.88 ± 5.73 min) in the study group was significantly shorter than that in the control group (30.38 ± 9.27min), the difference was statistically significant (t = -4.915, P <0.05). The number of rotary cutting knives in the study group (14.54 ± 6.35) was significantly less than that in the control group (21.81 ± 9.46), the difference was statistically significant (t = -3.254, P <0.05). Intraoperative bleeding rate of the study group was significantly lower than that of the control group, the difference was statistically significant (χ^2=6.933，P<0.05). Conclusion Preoperative ultrasound evaluation and precise positioning of breast masses to guide the formulation of the surgical plan, combined with the vacuum assisted minimally invasive rotation system via ultrasound leading to treat multiple masses of the breast, can shorten the operation time, reduce the number of rotary cutting knives and reduce intraoperative bleeding rate. It has certain clinical significance in achieving rapid surgery and true minimally invasive surgery.