| 摘要: |
| 目的 探寻ICAM-1在不同超声强度条件下联合脂氟显微泡辐照局部化疗肿瘤组织中表达水平的变化及其意义。方法 20只瘤兔随机分为以下4组：Control组不进行任何处理、CPMs组仅单纯瘤内注射卡铂缓释微球、S2000组使用西门子S2000诊断超声4V1探头联合微泡辐照局部化疗肿瘤组织、AP-170组使用澳洲AP-170超声治疗仪联合微泡辐照局部化疗肿瘤组织,各实验组分别使用不同强度超声联合脂氟显微泡辐照局部化疗肿瘤组织后,采用免疫组化及Western blot印迹法对肿瘤组织中ICAM-1表达水平进行检测。结果 ICAM-1 免疫组化半定量分析 Control组IOD 11038.62±873.54,CPMs组IOD12953.44±771.91,S2000组 IOD 17823.27±921.92,AP-170组IOD 23037.09±1175.46；ICAM-1 Western blot泳带分析Control组ICAM-1表达量为0.1851±0.0295,CPMs组0.2543±0.0354,S2000组0.4815±0.0391,AP-170组0.6195±0.0452。ICAM-1蛋白的表达水平在4组肿瘤组织中依次呈上升趋势,Control组与CPMs组比较差异无统计学意义,AP-170组与S2000组比较差异有统计学意义,与Control组、CPMs组比较差异有显著统计学意义。结论 强度较高的超声辐照使ICAM-1在肿瘤局部化疗微环境中呈高表达,对卡铂缓释微球介导的化疗药物在肿瘤细胞的增殖及转移等方面呈正反馈的影响。 |
| 关键词: ICAM-1,超声,肿瘤,微泡,卡铂缓释微球 |
| DOI： |
| 投稿时间：2018-05-02修订日期：2018-06-23 |
| 基金项目:国家自然基金面上项目(81371553) |
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| The changes of ICAM-1 expression in local chemotherapy tumor tissues under different ultrasonic irradiation combining microbubbles |
| YangXi,ZhuoZhongXiong |
| () |
| Abstract: |
| Objective To explore the changes of ICAM-1 expression in tumor tissues treated by local chemotherapy under different ultrasonic irradiation combing Zhifuxian microbubbles. Methods 20 tumor bearing rabbits were randomly divided into four groups: Control group, without any treatment; CPMs group, only intratumoral injection of carboplatin microspheres; S2000 group, diagnostic ultrasound irradiation on local chemotherapy tumor tissue using Siemens S2000 4v1 probe combining microbubbles; AP-170 group, the same as S2000 group except therapeutic ultrasound irradiation using Australian AP – 170. Different intensities of ultrasound were studied in the above treatment groups. Immunohistochemistry and Western blot were performed to assess the ICAM 1 expression in tumor tissues after treatment. Results Immunohistochemical semi-quantitative analysis of ICAM-1 showed that the IOD in Control group was 11038.62±873.54, while 12953.44±771.91 in CPMs group, 17823.27±921.92 in S2000 group, and 23037.09±1175.46 in ap-170 group. Western blot showed that the expression of ICAM-1 in the Control group was 0.1851±0.0295, while 0.2543±0.0354 in CPMs group, 0.4815 + 0.0391 in S2000 group, and 0.6195±0.0452 in ap-170 group. The expression level of ICAM 1 in the four groups exhibit an ascending trend in turn. No significant difference was found between the Control group and CPMs group. Compared with AP-170 group, S2000 group was statistically different, while control group and CPMs group were significantly different. Conclusion Higher intensity of ultrasonic irradiation gives rise to higher ICAM-1 expression in the local chemotherapy tumor microenvironment. In this condition, the chemotherapy drugs released by carboplatin sustained release microspheres would positively influence the proliferation and metastasis of tumor cells. |
| Key words: ICAM-1, ultrasound, tumor, microbubble, carboplatin sustained release microspheres |
