| 摘要: |
| 目的 研制一种可生物降解,其产物可完全排除机体的材料用于光声成像。方法 应用肉眼、光镜、电镜、激光粒径仪检测纳米粒物理特性；应用光声成像仪、红外光谱仪探讨纳米粒光声成像机制；应用光声成像仪检测纳米粒在体外、体内成像效果及安全性。结果 肉眼观察纳米粒透明乳状,无分层,分布均匀,粒径(230.9±54.58)nm,电位(-22.8±5.75)mv,稳定性较好。二氧化碳可能是纳米粒产生光声信号原因。纳米粒光声信号随浓度、温度、时间增加而增强、面积增大；体外激光辐照对细胞无损伤作用。在体内,经鼠尾静脉注入纳米粒各组正常裸鼠血氨浓度均较对照组升高(P<0.05),但精神体征与对照组无明显改变；肿瘤局部出现光声信号在4小时达到高峰,随后逐渐消失。激光能量对皮肤及皮下组织无损伤作用。结论 碳酸氢铵脂质纳米粒可用于光声成像,且是安全、有效、价廉、无副作用的。 |
| 关键词: 光声成像 产气 碳酸氢铵 纳米粒 造影剂 |
| DOI： |
| 投稿时间：2018-05-11修订日期：2018-05-29 |
| 基金项目:] 国家自然科学基金(81501481)。 |
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| A study of a novel gas-generating liposomal nanoparticle for photoacoustic imaging |
| XIA Ji-zhu,WANG Zhi-gang |
| (1.Institute of Ultrasound Imaging ﹠ Department of Ultrasound,The Second Affiliated Hospital of Chongqing Medical University; Chongqing Key Laboratory of Ultrasound Molecular Imaging) |
| Abstract: |
| Objective In this study, we have developed a biodegradable contrast agent for photoacoustic imaging. Its biodegradation products could be fully removed from a living organism. Methods Used light microscope、electron microscope、laser particle size analyzer to test general physical properties and observe stability. Results Nanoparticles were spherical with regular morphology and uniform size distribution, there was no obvious aggregation. The mean particle size of nanoparticles was (230.9±54.58) nm with uniform distribution and dispersion; Zeta potential was (-22.8±5.75) mV. CO2 may be the reason of producing photoacoustic signal. The intensity and the area of the photoacoustic signal enhance corresponding to the increase of concentration, temperature and time. There had no damage to cells by laser. During in vivo test, Serum ammonia concentrations for various treatment groups were higher than that for the control group, but vital signs observation of nude mice shows that there had no significant changes in foraging and movement with respect to the control group. photoacoustic signals were detected from the tumor, reached the peak in 4 h and then disappeared gradually. There was no damage to the skin and subcutaneous tissue by laser radiation. Conclusion It is a gas-generating nanoparticles of liposome encapsulating ammonium bicarbonate solution, and it is safe, effective, cheap and free of side effects. |
| Key words: Photoacoustic imaging Gas-generating Ammonium bicarbonate Nanoparticle contrast agent |
