Objective To investigate the diagnostic value of high frequency ultrasound and 99mTcO4-ectopic gastric mucosa imaging in children with Meckel diverticulum. Methods A retrospective study of the Meckel"s diverticulum was performed in our hospital from January 2017 to December 2017. The results of ultrasound and radionuclide imaging included a total of 13 cases. Analyze and compare the accuracy of high-frequency ultrasound imaging and radionuclide diagnosis of diverticulum. Results High-frequency ultrasound missed one case, two cases of misdiagnosis, and the accuracy was 76.92%; nuclide imaging missed one case, and 1 case was misdiagnosed, with an accuracy of 84.62%. However, there was no significant difference between the two methods (P > 0.05). Conclusion Both high-frequency ultrasound and radionuclide scan are helpful for the diagnosis of Meckel"s diverticulum, which can improve the diagnostic accuracy of diverticulum.