Aims to explore the application value of B-Learning based integrated teaching method on ultrasound lecture.Methods a total of 84 five-year clinical medical students were randomly divided into two groups (experimental group and control group), 42 in each group. According to the teaching plan of ultrasound medicine, the experimental group adopted the integrated teaching of B-Learning, and the control group adopted the traditional theory teaching in the classroom. Then we conducted a questionnaire survey and a written examination of ultrasound knowledge. A total of two teachers who didn"t participate in the study were blinded to go over examination papers. Scale test scores and examination results were analyzed by t test between two groups. Results the written examination scores were 85.1 + 15.2 points in the experimental group, 75.6 + 20.2 points in the control group, and the difference between the two groups was statistically significant (P<0.05). In the scale evaluation, 84 questionnaires (100%)were received.In total, the experiment group had score of 21.8±5.6, and the control group had score of 16.2±7.2(P<0.05)Conclusion the integrated teaching method based on B-Learning can improve the learning interest of the students in undergraduate teaching, and make the teaching methods diversified and the content of the course more easily understood. It has significantly improved the learning effect and strengthened the practical ability.