Objective To compare the contrast characteristics, diagnostic efficacy and consistency of contrast-enhanced ultrasound (CEUS) and enhanced CT (CECT) in the diagnosis of gallbladder space-occupying lesions. Methods Forty-eight patients with gallbladder space-occupying lesions were confirmed by operation and pathology. The enhancement characteristics of CEUS and CECT in 48 patients with gallbladder space-occupying lesions were analyzed. The sensitivity, specificity, accuracy, negative and positive predictive value of the two diagnoses were compared, and Kappa value of the consistency test were calculated. Results There was no significant difference in the enhanced characteristics of CEUS and CECT in gallbladder space-occupying lesions (P>0.05). The sensitivity of CEUS and CECT in the diagnosis of gallbladder lesions were 94.74% (18/19) and 89.47% (17/19) (P=0.895), respectively, and the specificities were 90.48% (19/21) and 95.24% (20/21) (P=0.905) , respectively, the accuracy was 92.50% (37/40) and 92.50% (37/40)（(P=1.00）, respectively, and the positive predictive values were 90.0% (18/20) and 94.44% (17/8), respectively，the negative predictive values were 95.0% (19/20) and 90.91% (20/22), respectively. Both CEUS and CECT were in good agreement with postoperative pathological findings (Kappa values were 0.850, 0.849, respectively), and the results of the two examinations were consistent (Kappa value = 0.900). Conclusion CEUS and CECT have similar features for the enhancement of gallbladder space-occupying lesions. The diagnostic results are consistent, and they all have high diagnostic efficacy.