Objective To evaluate the value of the outer gland volume of prostate (OPV) and the prostate outer gland volume specific antigen density (OPSAD) as non-invasive screening and diagnosis method for prostate cancer in a select population. Methods The diagnosis data of 221 subjects with serum PSA 4 to 10 ng/mL were analyzed. The prostate volume (PV), inner prostatic gland volume (IPV), and OPV were measured by transrectal ultrasound. Then the PSAD, prostate inner gland volume specific antigen density (IPSAD), and OPSAD were calculated. t test was used to compare the difference of the parameters between Pca and BPH. ROC curve was used to evaluate the diagnostic performance of OPV and OPSAD. Results The OPV and OPSAD of Pca group was (28.72±6.35) mL and (0.220±0.052) ng/mL_² respectively. The IPV and IPSAD of BPH group was (17.21±3.14) mL and (0.369±0.052) ng/mL_² respectively. There were significantly differences between the groups (P＜0.05). The IPV of Pca group was (15.58±8.34) mL, which was significantly smaller than OPV, and the IPSAD of Pca group was (0.373±0.04) ng/mL_², which was significantly higher than that of OPSAD s (P＜0.05). OPSAD was best for differentiate the Pca and BPH, the area under the curve was 0.900, the sensitivity was 89.83%, and the specificity was 85.48%. Conclusions The Pca patients were characterized with larger OPV and lower OPSAD. While the BPH patients were characterized with larger IPV and higher OPSAD. Pca patients had larger OPV than IPV and lower OPSAD than IPSAD. OPV and OPSAD had high value for differentiating the Pca and BPH.