| 摘要: |
| 目的 研究不同肝脏血流阻断方式对兔肝射频消融增强效果。方法 42只健康新西兰大白兔被平均分配到单纯消融组(NO组)、门静脉阻断联合射频消融组(PV组)、肝动脉门静脉全阻断联合射频消融组(HA+PV组)(n=14),射频系统采用RITA1500型冷循环射频消融仪,参数设定为15w、80℃、1min。按分组对各兔肝血管进行不同处理后,超声引导下进行射频消融。消融24h后超声造影测量消融灶各径线,取兔肝组织,对消融灶行大体测量,而后采用量筒排液法测量体积。各组另取3只实验兔分别于术前即刻、术后1、3、5、7日抽取兔肝动脉血检测ALT、AST、GGT、ALP。结果 1.三组间超声造影、大体标本测量消融灶范围均存在统计学差异,且HA+PV组消融灶最大。2.超声造影所测消融灶径线与大体标本相应测值比较未见明显统计学差异。3.三组实验兔ALT、AST均于术后第1日达到高峰,随后缓慢下降,术后7日基本恢复至术前即刻水平。各组间ALT、AST、ALP、GGT不同时间点变化趋势未见明显差异。结论 单独阻断门静脉血流或同时阻断门静脉、肝动脉血流均能有效增大射频消融灶各径线及体积,且全阻断效果最好。 |
| 关键词: 射频消融、血流阻断、肝脏、兔 |
| DOI： |
| 投稿时间：2018-12-12修订日期：2018-12-26 |
| 基金项目: |
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| Effect of hepatic-blood flow occlusion on liver radiofrequency ablation in rabbit model |
| HUANG Ye,LIU Zheng |
| (The Second Affiliated Hospital of Army Medical University,PLA) |
| Abstract: |
| ABSTRACT Objective To evaluate the impact of vascular occlusion of hepatic flow on radiofrequency ablation in rabbit model. Method 42 rabbits was assigned to one of three vascular occlusion groups , no occlusion(NO), portal vein (PV), both hepatic artery and portal vein (HA+PV), n=14.Radiofrequency lesions were created in vivo using RITA 1500 system with cool tip radiofrequency electrode, the parameters were set at 80℃, output at 15 W for 60 seconds. Implement different treatment to the vessels of liver according to the grouping. After the hepatic blood flow statement reached the expected protocol under colorful Doppler ultrasonography supervise, the radiofrequency ablation was then performed under real-time ultrasonography guidance. Using the CEUS to observe the morphology of the lesion and measure the size in three dimensions after 24h, then sacrificed the animals, find the biggest tangent plain of ablation zone in CEUS under B model ultrasonography guidance and cut the lesion out along this area, then measure the size of the ablation lesion. Take the atrial blood of three rabbit each group and test the AST,ALT,GGT,ALP in five time point (before therapy, and 1,3,5,7 days after therapy). Results 1. There is statistical difference between three groups in the size of ablation zone no matter under the CEUS or the gross specimen measurement, the ablation zone of the HA+PV group was the largest. 2. the diameters of ablation lesion measuring under CEUS has no significant difference with the specimen gross measurement. 3. the blood level of ALT, AST raised after treatment and get the peak value 1 day after therapy, then declined slowly. they almost back to the baseline 1 week later .There is no significant difference in AST,ALT,ALP,GGT test between different groups. Conclusion The vascular occlusion of hepatic inflow combined with radiofrequency ablation can enhance the efficiency of radiofrequency ablation especially when completely block the hepatic blood inflow. |
| Key words: Radiofrequency ablation Vascular occlusion Liver Rabbit |
