ABSTRACT Objective To evaluate the impact of vascular occlusion of hepatic flow on radiofrequency ablation in rabbit model. Method 42 rabbits was assigned to one of three vascular occlusion groups , no occlusion(NO), portal vein (PV), both hepatic artery and portal vein (HA+PV), n=14.Radiofrequency lesions were created in vivo using RITA 1500 system with cool tip radiofrequency electrode, the parameters were set at 80℃, output at 15 W for 60 seconds. Implement different treatment to the vessels of liver according to the grouping. After the hepatic blood flow statement reached the expected protocol under colorful Doppler ultrasonography supervise, the radiofrequency ablation was then performed under real-time ultrasonography guidance. Using the CEUS to observe the morphology of the lesion and measure the size in three dimensions after 24h, then sacrificed the animals, find the biggest tangent plain of ablation zone in CEUS under B model ultrasonography guidance and cut the lesion out along this area, then measure the size of the ablation lesion. Take the atrial blood of three rabbit each group and test the AST,ALT,GGT,ALP in five time point (before therapy, and 1,3,5,7 days after therapy). Results 1. There is statistical difference between three groups in the size of ablation zone no matter under the CEUS or the gross specimen measurement, the ablation zone of the HA+PV group was the largest. 2. the diameters of ablation lesion measuring under CEUS has no significant difference with the specimen gross measurement. 3. the blood level of ALT, AST raised after treatment and get the peak value 1 day after therapy, then declined slowly. they almost back to the baseline 1 week later .There is no significant difference in AST,ALT,ALP,GGT test between different groups. Conclusion The vascular occlusion of hepatic inflow combined with radiofrequency ablation can enhance the efficiency of radiofrequency ablation especially when completely block the hepatic blood inflow.