[Abstract] Objective:To study the therapeutic effect of pcDNA3-bFGF-NGF gene transfection mediated by ultrasound destruction on wound surface of diabetic rats . Materials and methods:Firstly, phospholipid, glycerol and perfluoropropane were mixed to form nano-bubbles. The bFGF and NGF gene plasmids were adsorbed on the surface of the nanospheres by electrostatic interaction, and then the lipid nanospheres carrying pcDNA3-bFGF-NGF gene were obtained, and their basic properties were tested. MTT method was used to observe its effect on cell activity. To observe the effect of ultrasound gene transfection instrument on the development of gene-loaded nano-bubble-enhanced ultrasound. Study on localization and release of pcDNA3-bFGF-NGF gene loaded by ultrasound、Enhanced transfection and the therapeutic effect on the wound of rats. Results: The prepared pcDNA3-bFGF-NGF nanospheres were spherical, uniform in size, with a particle size of about 450 nm and a surface potential of 12.7 MV. It had good biocompatibility and had no obvious toxicity to cells. Gene-loaded nanospheres can Significantly enhanced ultrasound imaging，after being destroyed by ultrasonic gene transfection instrument, the gene can be located and released, and the efficiency of gene transfection is obviously improved, which has a good therapeutic effect on the wound of rats. Conclusion: The ultrasonic destruction of the nanobubbles can significantly improve the transfection efficiency of the pcDNA3-bFGF-NGF gene, and can provide a new means for treating the diabetic wound. [Key words] Nano-bubbles, Plasmid, Gene transfection,Protein production,Echography