| 摘要: |
| 摘要：
目的：研究超声破坏纳泡介导pcDNA3-bFGF-NGF基因转染对糖尿病大鼠创面的治疗作用。
方法：首先将磷脂、甘油、全氟丙烷等混合制备成纳米级气泡，将制备的bFGF和NGF的基因质粒通过静电作用吸附于纳泡表面，从而得到载pcDNA3-bFGF-NGF基因脂质纳泡，并检测其基本特性。采用MTT法观察其对细胞活性的影响。观察超声基因转染仪作用前后载基因纳泡增强超声显影情况，研究超声破坏载pcDNA3-bFGF-NGF基因纳泡定位释放、增强转染及对大鼠创面的治疗效果。
结果：所制得的载基因pcDNA3-bFGF-NGF纳泡呈球形，大小较均一，粒径约 450nm，表面电位12.7mV， 具有良好的生物相容性，对细胞无明显毒性。载基因纳泡能显著增强超声显影，经超声基因转染仪破坏后，可定位释放基因，且基因转染效率明显提高，对大鼠创面有较好的治疗效果。
结论：超声破坏纳泡可显著提高pcDNA3-bFGF-NGF基因转染效率，可为糖尿病创面治疗提供一种新手段。
关键词：纳泡，质粒，基因转染，蛋白生成，超声成像 |
| 关键词: 纳泡，质粒，基因转染，蛋白生成，超声成像 |
| DOI： |
| 投稿时间：2019-01-14修订日期：2019-04-28 |
| 基金项目:载pcDNA3-bFGF-NGF基因的超声纳泡促进糖尿病大鼠创面愈合的实验研究 |
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| Experimental study on the treatment of Diabetic Rat wounds by ultrasonic destruction of pcDNA3-bFGF-NGF Gene nano-bubbles |
| wanglihua |
| () |
| Abstract: |
| [Abstract]
Objective:To study the therapeutic effect of pcDNA3-bFGF-NGF gene transfection mediated by ultrasound destruction on wound surface of diabetic rats .
Materials and methods:Firstly, phospholipid, glycerol and perfluoropropane were mixed to form nano-bubbles. The bFGF and NGF gene plasmids were adsorbed on the surface of the nanospheres by electrostatic interaction, and then the lipid nanospheres carrying pcDNA3-bFGF-NGF gene were obtained, and their basic properties were tested. MTT method was used to observe its effect on cell activity. To observe the effect of ultrasound gene transfection instrument on the development of gene-loaded nano-bubble-enhanced ultrasound. Study on localization and release of pcDNA3-bFGF-NGF gene loaded by ultrasound、Enhanced transfection and the therapeutic effect on the wound of rats.
Results: The prepared pcDNA3-bFGF-NGF nanospheres were spherical, uniform in size, with a particle size of about 450 nm and a surface potential of 12.7 MV. It had good biocompatibility and had no obvious toxicity to cells. Gene-loaded nanospheres can Significantly enhanced ultrasound imaging，after being destroyed by ultrasonic gene transfection instrument, the gene can be located and released, and the efficiency of gene transfection is obviously improved, which has a good therapeutic effect on the wound of rats.
Conclusion: The ultrasonic destruction of the nanobubbles can significantly improve the transfection efficiency of the pcDNA3-bFGF-NGF gene, and can provide a new means for treating the diabetic wound.
[Key words] Nano-bubbles, Plasmid, Gene transfection,Protein production,Echography |
| Key words: Nano-bubbles, Plasmid, Gene transfection,Protein production,Echography |
