| 摘要: |
| 目的：建立树舌多糖长循环热敏脂质体的优选制备方法。方法：采用薄膜分散法、逆向蒸发法、复乳化-溶剂挥发法制备树舌多糖长循环热敏脂质体。对脂质体的粒径、zeta电位、包封率和载药量、稳定性以及形态和热敏特性进行表征。结果 采用薄膜分散法、乳化法溶剂挥发法、复乳化-溶剂挥发法制备的脂质体包封率分别为45.7%，80.8%，82.4%，采用Delsa Nano C纳米粒度及Zeta电位分析仪测定脂质体粒径为163.2±17.1nm、测定Zeta电位为-11.81±1.87mV，载药量为7.2%,体外稳定性良好，具有良好的热敏释药特性。结论 确立了复乳化-溶剂挥发法制备树舌多糖长循环热敏脂质体的处方和工艺，取得较高的包封率及稳定性，可作为制备的优选方法。 |
| 关键词: 树舌多糖长循环热敏脂质体 复乳化-溶剂剂挥发法 包封率,载药量 |
| DOI： |
| 投稿时间：2019-01-18修订日期：2019-02-11 |
| 基金项目:国家自然科学基金面上项目81671691 |
|
| Study on preparation and quality characterization of long circulating thermosensitive liposomes of Polysacharides from Ganoderma Lucidum by double emulsion solvent evaporation methodYUAN Jiani, LUO Wen*, HAN Liying, YANG Xiao, GU Fen, DING Lei, ZHOU Xiaodong, LIU Liwen |
| YUAN JIANI,LUO WEN |
| () |
| Abstract: |
| Objective: To establish the optimum preparation method of long circulating thermosensitive liposomes from Ganoderma lucidum polysaccharides. Methods: The long circulating thermosensitive liposomes of polysacharides from Ganoderma Lucidum were prepared by membrane dispersion, reverse evaporation and multiple emulsion solvent evaporation. The particle size, zeta potential, entrapment efficiency, drug loading, stability and morphology of liposomes were characterized. Results: The encapsulation efficiency of liposomes was 45.7%, 80.8%, 82.4%,
respectively, by membrane dispersion method, emulsification solvent evaporation method and
solvent evaporation method. The Delsa Nano C nanoparticles and Zeta potential analyzer were used
to determi`ne the size and Zeta potential of liposomes which were 163.2nm and -11.81mV, the drug loading was 7.2%, and the stability in vitro was well and has good properties of thermosensitive drug releasing. Conclusion: The formulation and process of long cycle thermosensitive liposomes with compound emulsification and solvent evaporation have been established, and the high encapsulation efficiency and stability were obtained, which can be used as the preferred method for preparation. |
| Key words: Ganoderma lucidum polysaccharides, long circulating thermosensitive liposomes, double emulsion solvent evaporation method, entrapment efficiency, drug loading |
