Objective: To establish the optimum preparation method of long circulating thermosensitive liposomes from Ganoderma lucidum polysaccharides. Methods: The long circulating thermosensitive liposomes of polysacharides from Ganoderma Lucidum were prepared by membrane dispersion, reverse evaporation and multiple emulsion solvent evaporation. The particle size, zeta potential, entrapment efficiency, drug loading, stability and morphology of liposomes were characterized. Results: The encapsulation efficiency of liposomes was 45.7%, 80.8%, 82.4%, respectively, by membrane dispersion method, emulsification solvent evaporation method and solvent evaporation method. The Delsa Nano C nanoparticles and Zeta potential analyzer were used to determi`ne the size and Zeta potential of liposomes which were 163.2nm and -11.81mV, the drug loading was 7.2%, and the stability in vitro was well and has good properties of thermosensitive drug releasing. Conclusion: The formulation and process of long cycle thermosensitive liposomes with compound emulsification and solvent evaporation have been established, and the high encapsulation efficiency and stability were obtained, which can be used as the preferred method for preparation.