【】Objective To evaluate left ventricular (LV)systolic function in clinically stable heart transplantation patients by two-dimensional speckle tracking imaging (2D-STI) to explore the difference of left ventricular strain parameters between "healthy" transplanted heart and normal heart,providing a reference for monitoring rejection after transplantation by 2D-STE . Methods A total of 50 patients with no significant rejection after long-term clinical follow-up in our hospital were enrolled as a heart transplantation group (group A) and 30 healthy volunteers as a control group (group B). Two-dimensional echocardiography was performed. The strain parameters by EchoPAC analysis software included global longitudinal strain (GLS), GLS of endocardial and epicardial layers (endoGLS and epiGLS) and global circumferential strain (GCS), GCS of endocardial and epicardial layers(endoGCS and epiGCS), global radial strain (GRS).Results Compared with the normal control group, longitudinal strain parameters GLS, endoGLS, epiGLS, circumferential strain parameters endoGCS, GCS, epiGCS and radial strain parameters GRS were all decreased (P<0.001), and the difference was statistically significant.Conclusions Left ventricular systolic function in "healthy" heart transplant patients is still different from normal people. Exploring the range of left ventricular strain parameters that are more suitable for heart transplant patients can lay a foundation for 2D-STI technology to monitor postoperative rejection.