| 摘要: |
| 目的 探究高强度聚焦超声（HIFU）对大鼠实体结核动物模型的损伤效应。方法 通过在SD大鼠臀部皮下注射耻垢分枝杆菌（MS）悬液1 ml，将10只成功建立感染MS结核模型的SD大鼠分为HIFU组和对照组，每组5只，HIFU组进行超声辐照15 s（频率1 MHz，声强6369 W/cm2），对照组进行假照15 s（频率1 MHz，声强0 W/cm2）。观察两组辐照前后结节B超灰度变化。对结节组织分别进行HE染色和抗酸染色，光学显微镜下观察结节组织病理变化；激光共聚焦及扫描电镜下观察HIFU辐照损伤效应。结果 HIFU组经超声辐照前后SD大鼠结核结节B超灰度值比较差异有统计学意义（P=0.004）。HE染色：对照组光学显微镜下见皮肤组织结构完整，皮肤附件结构正常，未发现明显的炎症浸润，HIFU组光学显微镜下可以看到皮肤组织结构完整，真皮层组织未见损伤，皮肤附件正常。炎性细胞浸润加重，呈现急性损伤表现。抗酸染色：对照组中MS聚集成团，HIFU组中MS较分散、呈短棒状。与对照组比较，HIFU治疗组MS菌存活率显著降低，由93.613%降至48.596%。经HIFU辐照后，MS菌体表面变得粗糙，菌体细胞壁破裂。结论 一定剂量（辐照声强6369 W/cm2）的HIFU能有效抑制SD大鼠感染的MS结核结节，有望成为治疗结核病的新方法。 |
| 关键词: 高强度聚焦超声 耻垢分枝杆菌 损伤效应 结核病 鼠 |
| DOI： |
| 投稿时间：2019-03-28修订日期：2019-06-08 |
| 基金项目: |
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| Study on the damage effect of high intensity focused ultrasound on mycobacterium smegmatis lesions in SD rats |
| lijianhu,duyonghong |
| () |
| Abstract: |
| Objective To investigate the injury effect of high intensity focused ultrasound (HIFU) on solid tuberculous rat models..Methods By subcutaneous injection of 1 mL of Mycobacterium smegmatis (MS) suspension in the hip of SD rats, 10 successfully established MS TB infection model of SD rats were divided into HIFU group and control group, each group 5, HIFU treatment group was irradiated for 15 s ( frequency of 1 MHz , sound intensity of 6369 W/cm2) while control group for 15 s ( frequency of 1 MHz , sound intensity of 0 W/cm2).The changes of B-mode ultrasonic grayscale of nodules before and after irradiation were observed in the two groups. HE staining and acid-fast staining were used to observe the pathological changes of nodule tissues under optical microscope.The effects of HIFU irradiation damage were observed by confocal laser and scanning electron microscope.Results There was a statistically significant difference in the B - ultrasound grayscale of the tuberculous nodules in the HIFU group (P=0.004).HE staining: under the optical microscope, the skin tissue structure was intact and the skin attachment structure was normal in the control group. No obvious inflammatory infiltration was found. The structure of the skin tissue was intact under the optical microscope of the HIFU group, no damage was observed to the dermal tissue, and the skin appendages were normal.Inflammatory cells infiltrate and present acute injury. Acid-fast staining: MS in the control group was clustered, while MS in the HIFU group was dispersed and appeared as a short rod. Compared with the control group, the survival rate of MS bacteria in HIFU treatment group decreased from 93.613% to 48.596% significantly. After HIFU irradiation, the surface of MS cell became rough and the cell wall of the cell was broken. Conclusion HIFU at a certain dose (irradiation intensity 6369 W/cm2) can effectively inhibit the infected MS tuberculosis nodules in SD rats, which is expected to be a new method for the treatment of tuberculosis. |
| Key words: high intensity focused ultrasound Mycobacterium smegmatis Damage effect Tuberculosis（TB） The rat |
