Objective To explore whether the targeted microbubbles carrying adenovirus vector could delivery exogenous gene into brain gliomas under the destruction of ultrasound. Methods Making glioma models by transplanting glioma cells into mouse brains； making liposome microbubbles coupling RGD and carrying adenovirus vector, and testing the particle diameters and the ability of carrying gene and coupling RGD of these microbubbles; dividing animals into 3 groups, the animals in GroupⅠwere injected untargeted microbubbles without carrying adenovirus, the animals in GroupⅡ were injected untargeted microbubbles carrying adenovirus, the animals in Group Ⅲ were injected targeted microbubbles carrying adenovirus, all three groups were irriated by ultrasound; assaying the fluorescence intensity of mouse brains in three groups by small animal living fluorescence imaging system. Results The mouse brain gliomas showed the typical characters of human gliomas; the diameters of liposome microbubbles were about 1.10～1.20μm, and the concentration was about （7.8±0.43）×108/ml, these microbubbles could carry adenovirus and coulpe RGD; comparing with GroupⅠ and GroupⅡ , the fluorescence intensity were more obvious in GroupⅢ（P＜0.01）. Conclusion Destroying the targeted microbubbles carrying adenovirus could effectively delivery exogenous genes into the mouse brain gliomas under low frequency focus ultrasound.