摘要: |
目的 探讨载腺病毒靶向微泡在低频聚焦超声辐照下对颅内胶质瘤的靶向转染能力。 方法 原位接种U251胶质瘤细胞制备小鼠颅内胶质瘤模型;制备可以携载腺病毒载体和偶联RGD肽的脂质体微泡,并检测脂质体微泡的性状;实验动物分为3组,Ⅰ组为注射未携带腺病毒的非靶向微泡(对照组),Ⅱ组注射载腺病毒的非靶向微泡,Ⅲ组注射载腺病毒的靶向微泡,3组在注射微泡后接受超声辐照;利用小动物活体荧光成像系统观察和分析3组动物颅内荧光强度。结果 原位小鼠颅内胶质瘤模型符合临床胶质瘤影像学和病理学特征;制备的微泡直径在1.10~1.20μm之间,平均浓度为(7.8±0.43)×108/ml,既可以携带腺病毒,又可以链接RGD肽;小动物活体荧光成像显示,与Ⅰ组和Ⅱ组相比,Ⅲ组小鼠颅内荧光强度显著增强(P<0.01)。结论 静脉注射偶联RGD肽的载腺病毒靶向脂质体微泡可以将携载的目的基因有效地转染颅内胶质瘤。 |
关键词: 胶质瘤 靶向微泡 腺病毒 RGD肽 低频聚焦超声 |
DOI: |
投稿时间:2019-04-01修订日期:2019-04-23 |
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目) |
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Study of deliverying the exogenous genes into brain gliomas by destructing the targeted microbubbles carrying adenovirus vector under ultrasound irritation |
XU Zhongye |
(The department of neurosurgery,Hui zhou Third People’s Hospital,Huizhou City,Guangdong Province) |
Abstract: |
Objective To explore whether the targeted microbubbles carrying adenovirus vector could delivery exogenous gene into brain gliomas under the destruction of ultrasound. Methods Making glioma models by transplanting glioma cells into mouse brains; making liposome microbubbles coupling RGD and carrying adenovirus vector, and testing the particle diameters and the ability of carrying gene and coupling RGD of these microbubbles; dividing animals into 3 groups, the animals in GroupⅠwere injected untargeted microbubbles without carrying adenovirus, the animals in GroupⅡ were injected untargeted microbubbles carrying adenovirus, the animals in Group Ⅲ were injected targeted microbubbles carrying adenovirus, all three groups were irriated by ultrasound; assaying the fluorescence intensity of mouse brains in three groups by small animal living fluorescence imaging system. Results The mouse brain gliomas showed the typical characters of human gliomas; the diameters of liposome microbubbles were about 1.10~1.20μm, and the concentration was about (7.8±0.43)×108/ml, these microbubbles could carry adenovirus and coulpe RGD; comparing with GroupⅠ and GroupⅡ , the fluorescence intensity were more obvious in GroupⅢ(P<0.01). Conclusion Destroying the targeted microbubbles carrying adenovirus could effectively delivery exogenous genes into the mouse brain gliomas under low frequency focus ultrasound. |
Key words: Glioma Targeted microbubbles Adenovirus RGD Low frenquency focused ultrasound |