Objective To evaluate left ventricular systolic function in hemodialysis patients with valvular calcification(VC) by three-dimensional speckle tracking imaging(3D-STI). Methods Fifty-five hemodialysis patients with normal left ventricular ejection fraction (LVEF) were divided into VC group (26 cases) and NVC group (29 cases). 30 healthy volunteers were used as control group. All groups were performed two and three dimensional imaging. LVPWd, IVSd, LVESD ,LVEDD , LVEF, GLS, GCS, GAS , GRS and LVM were measured with 3D-STI . LVMI was calculated. Results Compared with the control group, IVSd, LVPWd, LVMI in hemodialysis group significantly increased(P<0.05), GLS、GCS、GAS and GRS significantly decreased (P<0.05), but LVEDD, LVESD and LVEF had no significant difference(P>0.05). Compared with NVC group, IVSd,LVPWd , and LVMI significantly increased(P<0.05), while GLS、GCS、GAS and GRS significantly decreased in VC group(P<0.05). Conclusion Cardiac systolic function was