| 摘要: |
| 目的 研究聚焦超声同步辐照对聚桂醇消融正常兔肝脏的增效作用。方法 健康新西兰大白兔26只随机分为三组：聚焦超声组（FUS组，6只）、聚桂醇组（LAU组，10只）及聚焦超声+聚桂醇组（FUS+LAU组，10只），分别给予聚焦超声辐照、聚桂醇及聚桂醇联合聚焦超声同步辐照处理。FUS焦点的平均声强（ISPTA）30.0W/cm2，工作占空比50%。48小时后观察处理区解剖及超声造影表现，测量血流充盈缺损区最大径。采用量筒法测定消融体积，消融灶HE染色后镜下观察组织学改变。采用Wilcoxon秩和检验比较LAU组与FUS+LAU组肝脏充盈缺损区最大径及消融体积的差异。结果 FUS组实验兔肝脏处理区未出现消融灶，LAU组与FUS+LAU组形成相似的黄白色消融灶。两组兔肝超声造影充盈缺损区平均最大径分别为(0.73±0.15)mm、(0.91±0.16)mm，差异有统计学意义(Z=-2.077，P=0.038)；两组平均消融体积分别为(0.19±0.06)ml、(0.47±0.20)ml，差异有显著统计学意义(Z=-3.182，P=0.001)，FUS+LAU组平均消融体积约为LAU组的2.5倍。两组消融灶HE染色均表现为典型的肝细胞坏死性改变。结论 聚焦超声同步辐照对聚桂醇消融正常兔肝有增效作用。 |
| 关键词: 聚焦超声 聚桂醇 消融 增效 |
| DOI： |
| 投稿时间：2019-05-05修订日期：2019-05-16 |
| 基金项目:国家重点研发计划(2017YFC0107300) |
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| Synergistic Effects of Focused Ultrasound Enhanced Lauromacrogol in Ablation of Rabbit Liver |
| YANG Jun,LI Ningshan |
| (Department of Ultrasound,the Second Affiliated Hospital of Army Medical University) |
| Abstract: |
| Objective To investigate the synergistic effects of focused ultrasound (FUS) on normal rabbit liver ablation with lauromacrogol (LAU). Methods Twenty-six healthy New Zealand rabbits were randomized to three groups: FUS group(n=6), LAU group(n=10), FUS+LAU group(n=10), which were given FUS exposure, lauromacrogol injection，lauromacrogol injection combined with synchronous FUS exposure respectively. The spatial peak time average intensity(ISPTA) of FUS focus is 30.0W/cm2, with a duty cycle of 50%. Forty-eight hours later, the performance of anatomic and contrast-enhanced ultrasonography of the processing area was observed，and the largest diameter of filling defect was measured. The ablation volume was measured by graduated cylinder, and the histological changes were observed through HE stain. Wilcoxon rank sum test was applied to compare the difference of the largest diameter as well as ablation volume between LAU group and FUS+LAU group. Results Liver ablation was not found in FUS group, while that was found in both LAU group and FUS+LAU group with similar performance. The average largest diameter of LAU group and FUS+LAU group were (0.73±0.15)mm and (0.91±0.16)mm respectively，which showed significant difference(Z=-2.077，P=0.038). The average ablation volume were (0.19±0.06)ml and (0.47±0.20)ml respectively, which showed significant difference (Z=-3.182，P=0.001). The average ablation volume of FUS+LAU group was about 2.5 times that of LAU group. Hepatocyte necrosis was typical in both groups. Conclusion The synchronous application of focused ultrasound enhanced the ablation effect of lauromacrogol on rabbit liver and produced obvious synergistic effect. |
| Key words: Focused Ultrasound Lauromacrogol Ablation Synergize |
