Objective:To compare the diagnostic value of three-dimensional contrast-enhanced tomographic ultrasound imaging(3D-CETRUS TUI) and multiparametric magnetic resonance imaging(mp-MRI) in patients with different serum prostate cancer specific antigen (PSA) level. Methods: 131 patients with suspected prostate cancer in our hospital from April 2017 to October 2018 were included in this prospective study. All cases underwent 3D-CETRUS TUI and mp-MRI examination and transperineal prostate 12 needle system puncture under the guidance of transrectal ultrasound. According to the gold standard of puncture pathological results, three different PSA level groups (4 < PSA≤10 ng/ml、1020ng/ml) were analyzed. Results: The overall sensitivity of 3D-CETRUS TUI and mp-MRI in the diagnosis of prostate cancer was 78.05%, 84.10%, and the overall specificity was 77.55% and 65.30%. The sensitivity of 3D-CETRUS TUI and mp-MR in the diagnosis of prostate cancer were 64.40%, 78.72% and 79.40%, 89.33% respectively in the range 4 20 ng/ml group, both methods were correctly diagnosed. Conclusion:In some PSA levels, 3D-CETRUS TUI can obtain similar diagnostic conclusions as mp-MRI, and it is more rapid and efficient, so it is expected to become another important imaging diagnosis method for the diagnosis and evaluation of prostate cancer.