[Abstract] Objective To study the changes of cardiac synchronization and its clinical significance in patients with left ventricular false tendons(LVFT) using routine two-dimensional and real-time three-dimensional echocardiography(RT-3DE).Methods 173 patients confirmed by echocardiography, aged 36.7±8.44 years old and 23 females, were detected continuously in our hospital from January 2016 to June 2018 as the LVFT group, and 150 cases without LVFT, aged 35.84±7.53 years old and 20 females, were selected as the control group during the same period of physical examination according to gender and age matching. Two-dimensional echocardiography recorded the size, connection mode and direction variation of the patient''s LVFT in detail, and pulse doppler and real-time three-dimensional echocardiography evaluated the left atrioventricular, interventricular and left ventricular synchronization, then compared the difference of synchronicity between the two groups and its correlation with the morphology of LVFT. Results There were statistically significant differences in left ventricular dyssynchrony between LVFT patients and those without (P<0.05), and further classification of LVFT''s size, connection mode and direction variation showed that the connection mode was independently related to standard deviation of time to minimum systolic volume in left ventricle 16 segments（TMSV16-SD）by multiple regression (P<0.05).Conclusion The connection mode of LVFT was an important factor which affecting left ventricular synchronization, so it has good clinical reference value.